Population genetics is the study of how localized groups of individuals capable of interbreeding and creating fertile progeny change genetically over time. The Hardy-Weinberg equilibrium accounts for gene pools that do not change genetically over time. In this experiment, I intended to determine whether the sample population consisting of my fellow biology lab classmates would fall in the Hardy-Weinberg equilibrium with respect to the ALU insert from human chromosome 8. My hypothesis was that this sample population would fall in the Hardy-Weinberg equilibrium with respect to the ALU insert. To analyze whether this sample population was in the Hardy-Weinberg equilibrium or not, I amplified a sample DNA polymerase from a cheek cell of each individual through PCR and electrophoresis. I then determined the allele frequency and predicted the genotype frequencies from these allele frequencies through the Hardy-Weinberg equation. Based on the chi-squared test, I found that the sample population did fall in the Hardy-Weinberg equilibrium, as my p-value was greater than .05. This is reasonable since conditions for the Hardy-Weinberg equilibrium appear to be met: inbreeding is infrequent, populations outside of our sample population have allele frequencies resembling those inside our sample population, mutation rate for the ALU insert is minimal, and selection occurs only against rare homozygotes.
Population genetics is defined as the research and analysis of a gene pool in a population. A gene pool is the configuration of the sum of the alleles of each individual in a population. A comparison of the genotype frequencies from one generation to another indicates whether evolution has occurred. Gene pools that are not evolving are said to be in the Hardy-Weinberg equilibrium (Campbell 456). The main objective of this human population genetics experiment was to examine the allele frequencies for the sample population of my biology class and predict genotype frequencies. I wanted to calculate the proportion of individuals in the sample population with ALU inserts to determine whether the insert is in the Hardy-Weinberg equilibrium. ALU inserts are small, repetitive sequences of DNA distributed through the genomes of primates. ALU inserts from human chromosome 8 of the tissue plasminogen activator (TPA) gene were selected because they are unwavering and reliable genetic markers, as most of them show no signs of being subject to disappearance or repositioning (Batzer 12288). My hypothesis was that the ALU insert would be in the Hardy-Weinberg equilibrium because the five assumptions for the equilibrium of random mating, a large population, and no selection, mutation or migration seem to correlate with respect to the ALU insert in this random and diverse sample population.
Materials and Methods
In order to accomplish the above objectives, a lab technique known as PCR, or polymerase chain reaction, was used to amplify DNA sequences and thus genotype this sample population. To perform PCR on the sample population, a sample quantity of the template DNA was taken from each individual by use of a sterilized cotton swab placed inside the cheek and dabbed around a few times to extract cheek cells. The cotton swab was then swirled around a few times in a 1.5 ml microcentrifuge tube with 500 µL of 5% Chelex solution. The tube was then capped, placed in a 100° C heating block for 10 min., carefully removed and cooled on ice for 1 min., spun for 2 min. in a small microcentrifuge, and then finally 20 µL of the supernatant liquid was pipetted into a 0.5 ml tube. The template DNA was then left on ice while each of us set up our PCR mixture, which was a buffered solution consisting of primers (the DNA strands that are complimentary to the ends of the particular DNA sequence), Taq polymerase (the most regularly used heat-stable polymerase), dNTP’s (the four building blocks of DNA), and the added cofactor of Mg2+. In a new...
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