Hplc Coke Analysis
HPLC Determination of Aspartame in Diet Coke
Name: xx
Student Number: xxx
Class: xxx
Aim
The aim of this experiment was to determine the concentration of aspartame in a sample of diet Coke® using HPLC as investigation tool.
Introduction
High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques of nonvolatile organic compounds. Chromatographic processes can be described generally as mass-transfer between stationary and mobile phase. In HPLC, a liquid mobile phase is used to separate individual components of complex mixtures. Stationary phase can be either liquid or solid (like that in experiment).
The standard design of HPLC involves a core of four to five functional elements. These elements are: a filtered solvent reservoir of mobile phase and degassing system, the pump, an injector, a column (optionally housed in an oven) and detector/data system. Test mixtures are dissolved in solvent and micro injections are forced to flow under a high pressure through the column. In the column, the mixture separates into its components and these are detected and recorded on chromatogram. The resolution of various components is determined by extent of interaction between the solute components and the stationary phase. The interaction of the solute with mobile and stationary phases can be manipulated through different choices of both solvents and stationary phases. As a result, HPLC acquires a high degree of versatility not found in other chromatographic systems and it has the ability to easily separate a wide variety of chemical mixtures.1
[pic]
Diagram 1: Typical HPLC Design
From: http://www.comsol.com/stories/waters_corp_hplc_systems/full/
Individual Modules of HPLC Instrument
The modular HPLC unit in the experiment was essentially composed of an isocratic pump, an auto sample injector, a thermostatically controlled column, a
Name: xx
Student Number: xxx
Class: xxx
Aim
The aim of this experiment was to determine the concentration of aspartame in a sample of diet Coke® using HPLC as investigation tool.
Introduction
High Performance Liquid Chromatography (HPLC) is one of the most widely used analytical techniques of nonvolatile organic compounds. Chromatographic processes can be described generally as mass-transfer between stationary and mobile phase. In HPLC, a liquid mobile phase is used to separate individual components of complex mixtures. Stationary phase can be either liquid or solid (like that in experiment).
The standard design of HPLC involves a core of four to five functional elements. These elements are: a filtered solvent reservoir of mobile phase and degassing system, the pump, an injector, a column (optionally housed in an oven) and detector/data system. Test mixtures are dissolved in solvent and micro injections are forced to flow under a high pressure through the column. In the column, the mixture separates into its components and these are detected and recorded on chromatogram. The resolution of various components is determined by extent of interaction between the solute components and the stationary phase. The interaction of the solute with mobile and stationary phases can be manipulated through different choices of both solvents and stationary phases. As a result, HPLC acquires a high degree of versatility not found in other chromatographic systems and it has the ability to easily separate a wide variety of chemical mixtures.1
[pic]
Diagram 1: Typical HPLC Design
From: http://www.comsol.com/stories/waters_corp_hplc_systems/full/
Individual Modules of HPLC Instrument
The modular HPLC unit in the experiment was essentially composed of an isocratic pump, an auto sample injector, a thermostatically controlled column, a