HPLC Analysis of Over –The –Counter Naproxen in Alleve Tablets Eric Sorenson
Work Completed On 11/7/12
This analysis of Naproxen in pharmaceutical grade Alleve tablets consisted of a number of tasks and activities necessary for the accurate measurement of Naproxen content. Aside from setting up and operating the HPLC Shimadzu 1 instrument, all of the standards and samples had to be prepared by the operator. This entire lab was performed in the morning of 11/7/12. The first task was to prepare a naproxen standard from chemical grade pure naproxen compound. A mass of 24.4 mg of naproxen was weighed out on the analytical balance, and diluted to 0.976 mg/mL with 25mL of a solution of 40% ACN/60% water. This standard naproxen solution was further diluted to a concentration of 0.0488 mg/mL which was then used to do a system suitability test. The HPLC conditions were as follows; Xterra 5.0 cm x 0.46 cm column with 3.5 µm C18 particles, mobile phase was 50% water/ 50% ACN, injection volume was 10 µL of sample, run time of 5 minutes, flow rate of 1.0 mL/minute, and a detector at 254 nm. The average retention time of this standard after five system suitability tests was 1.335 minutes, and the average peak was 158,442.75. The relative standard deviations for both of these were < 1%. This is a good system suitability test, indicating that it is reproducible. The next task was to prepare a Naproxen calibration standard curve. A series of naproxen standards were prepared ranging from 40 to 60 µg/mL increasing by 5 µg/mL. This was prepared using a secondary naproxen standard 0.1 mg/mL. These five naproxen standards including one blank sample were injected into the HPLC with the same conditions as the previously described. The results were used to construct a calibration curve using excel via linear regression analysis, see figure 1. The equation of the best fit line was eventually used to calculate the concentration of the Alleve tablets. Three Alleve...
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