How to Design a Lab in Biology Ib

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Design

Research question:
This should be a clear focused question that says exactly what you are investigating. It shouldn't be too long and it must include the dependent and independent variables. Eg. What is the effect of pH on the activity rate of salivary amylase? Dependent variable: activity rate

Independent variable: pH

Hypothesis:
This is a paragraph or two where you explain your research question. You are going to say something like: "Salivary Amylase is a an enzyme that digests starch into di- and monosaccharides. Since it's a salivary amylase, the enzyme works best at an alkaline pH of 7, in other words, the optimum pH is 7. At this pH, the rate of amylase activity will be at it's highest. A pH that is much lower (very acidic) or much higher (very alkaline) will denature the enzyme permanently (specifically the active site), and the enzyme can't function anymore. The activity of the enzyme will decrease as we increase or decrease pH." You may also want to include a graph to show this if this possible.

Variables:
A list or a table that include:
-Independent variable: this is the variable you're changing. In the example above, the pH. -Dependent variable: this is what changes when you change the independent variable. Eg. Activity rate. -Controlled variables: these are all the other variables that must be kept the same in order to get an accurate results. For example, Temperature, pressure..etc.

Apparatus:
This is the list where you include everything you are going to use. Make sure you don't forget anything. My teacher always told me to include a diagram of the apparatus, so you may want to add that too. When listing the apparatus, be specific:

1)'A beaker' wont work, you have to specify the type and the volume. Same for any other apparatus of this sort. 2)When listing chemical substances like enzymes or starch solutions. Include the volume and the concentration. 3)For Solid substances used, include the mass in 'g'

4)When mentioning...
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