HISTOLOGY lab report

Topics: Muscle, Epithelium, Tissues Pages: 5 (1004 words) Published: May 30, 2015
HISTOLOGY

PURPOSE:
The purpose of this exercise is to be able to identify and correctly name the major tissue types in the body, as well as identifying the subcatergories of tissue types while observing them through the microscope and diagrams, and be able to explain the location and function of the tissue types in the body. There are not any real safety concerns for this lab other than making sure correct use and care of the microscope is used. EXERCISE 1: EPITHEILIAL TISSUE

Epithelial Tissue Observations
Tissue Type
Observations
Simple Squamous
Single layer of cells, flat in appearance
Simple Cuboidal
Cells appear to be squarelike, nuclei are in the middle of each cell, basement membrane, connective tissues Simple Columnar (stomach)
Tall (like a column), elongated nuclei, there are gastric pits visible, basement membrane, microvilli, connective tissue Simple Columnar (duodenum)
Columnar in appearance, microvilli are present as well as Brunner’s glands Stratified Squamous (non-keratinized)
Appear to have a mix of cuboidal and columnar cells in the basal layers, with squamous cells at the top. Pseudostratified Ciliated Columnar
Appears to have more than one layer of column of cells, but the cells appear to be resting on the basal lamina. Cilia are on the top of the cells. Transitional
Top cells appear to be larger, round, and have 2 nuclei. Connective tissue and a basement membrane Stratified Cuboidal (online)
Double layer of squarelike cells, each having their own nuclei Stratified Columnar (online)
Layers of cuboidal cells on the bottom with a single layer of columnar cells on the top.

Questions:
A. The study of histology is important in the overall understanding of anatomy and physiology so that you can gain an understanding of the structure and functions of different tissues throughout the body systems. B. Epithelial tissues are named by their shape and cell arrangement. C. Some epithelial tissues are stratified because there are many layers of cells to help protect areas that are known for lots of wear and tear. These types of tissues are generally found on the skin and mouth cavity. D. The functions that are supported by having cuboidal and columnar cells having large, open cytoplasm include absorption, secretion, and allowance for rapid diffusion or transport of substances through the basement membrane. E. Identification of epithelium (see lab manual page 70 for drawings) a. 1 = simple squamous

b. 2=simple columnar
c. 3=pseudostratified columnar
d. 4= stratified squamous

EXERCISE 2: CONNECTIVE TISSUE
OBSERVATIONS:
Data Table 2: Connective Tissue
Tissue
Amount and Shape of Cells
Amount of Matrix
Are there fibers? If so, are they parallel or scattered?
Mesenchyme (online)
23-polygonal
20%
Yes, both parallel and scattered
Aerolar (online)
28- spindle shaped
30%
Yes, parallel
Adipose (online)
8-polygonal shaped
N/A
No
Dense Irregular (online)
20-polygonal shaped
35%
Yes, scattered
Reticular
40- starlike shaped
30%
Yes, scattered
Dense Regular: Tendon
Too many to count (TMTC)
N/A
Yes, parallel
Hyaline Cartilage
TMTC
30%
No
Elastic Cartilage
TMTC
50%
Yes, scattered
Fibrocartilage
TMTC
40%
Yes, parallel
Compact Bone
TMTC, round in shape
90%
Yes, parallel
Human Blood
TMTC
TMTC
No

Questions:
A. The primary function of connective tissue is to join bodily structures (bones, muscles, etc.) together and to hold tissues (muscles, tendons, organs, etc.) in place. B. The shape of a cell gives us insight into the function of the tissue (flat cells protect, large open cells store or secrete materials, etc.) C. The matrix is non-cellular material, usually made of protein, that provides support for certain tissues and acts as a filter between the tissue cells and the blood. D. Collagen fibers provide support, flexibility, and strength for the connective tissue. E. The limited blood supply of tendons, ligaments, and cartilage could...
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