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Health Is Greatest

By | July 2013
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PRELAB

Catalase in found in most organisms that undergo aerobic cellular respiration. Catalase functions mainly to prevent the accumulation of hydrogen peroxide in the body. Organelles called peroxisomes contain antioxidant enzymes, which act in concert to protect cells from damage cause by highly reactive oxygen species [e.g. hydrogen peroxide] that are generated during metabolism. Peroxisomes contain the enzymes catalase, D-amino catalase, urate oxidase, and alpha hydroxylic acid oxidase. The name peroxisome was applied because this organelle is specifically involved in the formation and decomposition of hydrogen peroxide. H2O2 and free radicals are produced as a result of certain oxidative reaction involved in the break down of amino acids and fats. These are very reactive chemical species that could damage cellular machinery. To protect the cell from these destructive by-products, such reactions are segregated within these small membrane-bound peroxisomes. Purpose:

How does the action of the biological enzyme catalase found in liver compare to that found in potato, and to a non-protein catalyst manganese dioxide. How do changes in substrate concentration, enzyme concentration, surface area and environmental factors such as temperature or pH affect the rate of enzyme activity?

Note that you will be studying the reaction where hydrogen peroxide is broken down into water and oxygen gas. For the rate of chemical reaction use a scale from 0-5 where 0(no bubbling) to 1(very little, slow bubbling) are the lowest rates and 5 is the maximum (lots of bubbling, quick).

2H2O2 2H2O + O2

Variables- something that can change or vary in an investigation.

The cause variable is the independent variable. The one thing you change consciously. The effect variable is the dependent variable. This depends on the variable you purposefully change. (Rate of reaction in this particular lab.) All other variables must be kept constant or controlled....
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