HAEMATOLOGICAL PARAMETERS OF CIRRHINUS MRIGALA (HAMILTON) FED WITH ALGAL FORMULATED DIET Muhammad Jamal Haider1, Ehsan Elahi Valeem2, Talat Saleem1, Syed Hasan Wahid1, Muhammad Shafiq1, Rukhsana Talat1 & Yasmin Majeed1 1 2
Department of Zoology, FUUAST, Gulshan-e-Iqbal Campus, Karachi-75300 Department of Botany, Govt. Degree College Buffer Zone, North Nazimabad Town, Karachi-75850
ABSTRACT One hundred fishes of Cirrhinus mrigala (Hamilton) were captured alive from Keenjhar Lake, district Thatta (Sindh) reared in captivity, trialled for algal versus controlled diets, blood samples assessed for evaluation of haematological and biochemical test parameters for both controlled and trialled sets. Altogether 30 different tests comprising of haematology (granulocytes and agranulocytes) and plasma as well as serum biochemistry (enzymes, proteins, elements and a steroid) were analyzed from blood samples. The test parameters will be useful for interpreting screening tests as multiple body systems can be involved in the growth processes. Fishes that fed with algal diet showed a higher feed intake rate as compared to the controlled diet. The fishes fed with algal diet showed a substantial improvement in almost all the growth parameters like body weight gain percentage, specific growth rate, feed conversion ratio, metabolic growth rate as compared to those fed with controlled diet during a trial period of four weeks. It is suggested that indigenous algae should be used in combination with the controlled diet to achieve improved results in commercial farming of valuable Pakistani major carps. Keywords: Algal diet, Cirrhinus mrigala, Haematology, Blood, Plasma, Thatta, Pakistan. INTRODUCTION White meet of fishes are considered as one of the important food sources for human beings as their flesh contains a high amount of calcium, phosphorus and proteins and low amount of cholesterol. Haematological parameters can give us quick assessment of fish health (Hickey 1976) that can be the best indicators for an experimental fish like Cirrhinus mrigala (Hamilton 1822). Changes in haematology are due to the environmental or physiological stress (Blaxhall 1973, McLeavy 1973, Chakrabarty & Banerjee 1988). C. mrigala is an edible fish of Pakistan, commonly known as major carp and locally named as Morakhi or Mori (Fig. 1). These fishes inhabit in different environmental conditions (Mirza 2003) like found in Keenjhar Lake, district Thatta (Sindh Province of Pakistan). Blood cells analysis gives us first hand information for finding and forecasting fish diseases (Anderson 2003). One hundred adult specimens of C. mrigala each weighing ca 500 g and 25 cm in length captured alive from Keenjhar Lake, equally distributed into two experimental ponds, reared in captivity, algal feeding trialled versus controlled diet. Blood samples of both controlled and trialled fishes assessed for evaluation of haematological and biochemical test parameters. The physiological and physiopathological evaluation of different biochemical and cellular constituents in blood of fishes is of fundamental importance because quantitative variations in blood and morphological parameters can be assessed by environmental factors and pollutants (Juneja & Mahajan 1983, Ranzani-Paiva et al. 1997). The haematological study is to be conducted frequently for the detection of physiopathological changes such as exposure to heavy metals in different stress conditions (Nussey et al. 1995). Therefore, morphological features like colour and length of the fish body were closely observed apart from analysis of complete blood picture.
Haider MJ, Valeem EE, Saleem T, Wahid SH, Shafiq M, Talat R & Majeed Y
Table I. Haematological parameters of Cirrhinus mrigala (Hamilton 1822) administered with controlled and algal diet in experimental ponds i.e. 1-4 weeks (values are mean ± SD, n = 10). Weeks S.
Test Parameters Haematology...