Grade 12 Bio - Enzyme Lab

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SBI 4U0: Enzyme Lab

Purpose: To compare the action of the enzyme catalase, to a non-protein catalyst under different conditions.

Observations:

| | |Observations |Rate of Reaction |Interpretations | |A |Sand |- Sand piled up at the bottom of |0 |- There is no reaction between sand and| | | |the test tube and no bubbles | |hydrogen peroxide, because sand does | | | |arose | |not contain any catalysts or enzymes to| | | | | |break down hydrogen peroxide | | |MnO2 |- Reaction occurred right away, |5 |- MnO2 acts like a catalyst which | | | |bubbles rose almost to the top of| |breaks down H2O2 into water and oxygen | | | |the tube | |gas with a lower activation energy | |B |Liver |- Reaction occurred right away, |4 |- Liver contains large amounts of the | | | |and big, white bubbles rose the | |enzyme catalase, which break down H2O2.| | | |top of the test tube | |This made the reaction occur quickly | | | | | |and form bubbles. | | |Potato |- Very little bubbles appeared |2 |- The reaction was very moderate and | | | |from the reaction, and did not | |did not occur quickly because potato | | | |rise very high in the tube or | |does not have a lot of the enzyme | | | |occur quickly | |catalase | |C |Used liver + Fresh liver |- Some bubbles formed and the |3 |- The reaction should have had a higher| | | |reaction was moderate | |rate of reaction, because the enzymes | | | | | |in the used liver are still active and | | | | | |can be used in the reaction again. The | | | | | |fresh liver can also perform this | | | | | |reaction with its catalase enzyme. Due | | | | | |to an error or impurity of equipment, | | | | | |the reaction was not as strong. | | |Used liver + H2O2 |- Reaction occurred quickly and a|4 |- The enzyme in the liver is still | | |...
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