Glucose Concentation Experiment (Lab Report)

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RESEARCH QUESTION
How does the glucose concentration effects the time taken for the potassium permanganate to decolourise from purple to colourless ?

HYPOTHESIS
The higher the glucose concentration the shorter the time taken for the potassium permanganate to decolourise from purple to colourless.

VARIABLES
VARIABLE| UNITS| RANGE| METHOD FOR CONTROL|
INDEPENDENT VARIABLE-Concentration of glucose solution| %| 5%10%15%20%25%30%| Different glucose concentration are tested| DEPENDENT VARIABLE-The time taken for the potassium permanganate to decolourise from purple to colourless| second (s) | -| Measure the time taken for the potassium permanganate solution to decolourise from purple to colourless by using stopwatch|

CONTROLLLED VARIABLE| UNIT| METHOD FOR CONTROL|
Concentration of sulphric acid| molarity (M)| Use the sulphric acid with the same concentration for the all sample| Volume of sulphric acid used to test each sample of glucose concentration| cm3| Use the same volume of sulphric acid to test each sample of glucose concentration that is 5cm3| Volume of potassium permanganate used to test each sample of glucose concentration| cm3| Use the same volume of potassium permanganate to test each sample of glucose concentration that is 2cm3| Volume of different glucose concentration used in every test| cm3| Use the same volume of different glucose concentration in every test that is 10cm3| The volume of unknown solution used in every test| cm3| Use the same volume of each unknown solution in every test that is 10cm3 |

APPARATUS AND MATERIAL
APPARATUS| QUANTITY|
Eye protection| 1|
Stopwatch| 2|
Boiling tube and rack| 9|
Beaker (100ml)| 3|
Measuring cylinder (50ml)| 1|
Label| 9|
Glass rod| 1|
Syringe | 3|

MATERIAL| QUANTITY|
Glucose solution with different concentration| 60cm3|
Sulphric acid (1M)| 45cm3|
Potassium permanganate| 18cm3|
3 unknown solution| 10cm3 for each solution|

METHODOLOGY
Refer to Student's Handbook for Biology HL (Year 1/2012) of College MARA Banting ~ Page 180

COLLECTIVE DATA
A.QUALITATIVE DATA
1. The initial colour of potassium permanganate is purple and it is odourless. 2. The colour of potassium permanganate change from purple to colourless after the reaction. 3. The glucose solution is colourless.

4. The sulphric acid is colourless.
5. The time taken for the potassium permanaganate to decolourise is absolutely different for every glucose concentration. 6. The colour of potassium permanganate does not change when the unknown solution is tested.

B.QUANTITATIVE DATA
Concentration of glucose (%)| The time taken for the potassium permanganate to decolourise from purple to colourless, t (s ± 0.1s)| 5| 123.0|
10| 66.0|
15| 44.0|
20| 37.0|
25| 29.0|
30| 23.0|
Unknown solution| |
A| 207.0|
B| 13.0|
C| No colour change|

TABLE 1 : The time taken for the potassium permanganate to decolourise from purple to colourless (my data)

Concentration of glucose (%)| The time taken for the potassium permanganate to decolourise from purple to colourless,t(s ± 0.1s)| Average of time taken,t(s ± 0.1)| Rate of reduction,1/t(s-1)| Uncertainty of rate of reduction,∆1/t(s-1)| | Trial 1| Trial 2| Trial 3| Trial 4| Trial 5| Trial 6| | | | 5| 155.5| 120.0| 120.7| 123.0| 117.0| 123.8| 126.7| 0.00789| 0.000006| 10| 92.0| 54.0| 69.0| 66.0| 57.0| 57.2| 65.9| 0.0152| 0.00002| 15| 60.2| 45.0| 45.7| 44.0| 46.0| 37.2| 46.4| 0.0216| 0.00002| 20| 43.6| 43.0| 46.1| 37.0| 45.0| 42.8| 42.9| 0.0233| 0.00005| 25| 40.7| 38.0| 43.6| 29.0| 44.0| 48.0| 40.6| 0.0247| 0.00006| 30| 33.8| 34.0| 41.6| 23.0| 41.0| 47.0| 36.7| 0.0272| 0.00007| Unknown solution| |

A| 163.9| 197.2| 233.6| 207.0| 175.0| 120.4| 182.9| 0.00546| 0.000003| B| 18.2| 11.8|...
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