Genetic Transformation

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GENETIC
TRANSFORMATION
Edited by María Alejandra Alvarez

Genetic Transformation
Edited by María Alejandra Alvarez

Published by InTech
Janeza Trdine 9, 51000 Rijeka, Croatia
Copyright © 2011 InTech
All chapters are Open Access articles distributed under the Creative Commons Non Commercial Share Alike Attribution 3.0 license, which permits to copy, distribute, transmit, and adapt the work in any medium, so long as the original work is properly cited. After this work has been published by InTech, authors have the right to republish it, in whole or part, in any publication of which they are the author, and to make other personal use of the work. Any republication, referencing or personal use of the work must explicitly identify the original source. Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published articles. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials, instructions, methods or ideas contained in the book. Publishing Process Manager Dragana Manestar

Technical Editor Teodora Smiljanic
Cover Designer Jan Hyrat
Image Copyright Sashkin, 2011. Used under license from Shutterstock.com First published August, 2011
Printed in Croatia
A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org

Genetic Transformation, Edited by María Alejandra Alvarez
p. cm.
ISBN 978-953-307-364-4

free online editions of InTech
Books and Journals can be found at
www.intechopen.com

Contents
Preface IX
Part 1

Agrobacterium: New Insights into a Natural Engineer 1

Chapter 1

Agrobacterium-Mediated Genetic Transformation:
History and Progress 3
Minliang Guo, Xiaowei Bian, Xiao Wu and Meixia Wu

Chapter 2

Structure-Function Analysis of Transformation Events
Yuri N. Zhuravlev and Vladik A. Avetisov

Part 2

29

Plant Transformation: Improving Quality of Fruits,
Crops and Trees - Molecular Farming 53

Chapter 3

Genetic Transformation in Tomato: Novel Tools
to Improve Fruit Quality and Pharmaceutical Production 55
Antonio Di Matteo, Maria Manuela Rigano,
Adriana Sacco, Luigi Frusciante and Amalia Barone

Chapter 4

Genetic Transformation Strategies in Fruit Crops
Humberto Prieto

Chapter 5

Citrus Transformation: Challenges and Prospects 101
Vicente Febres, Latanya Fisher, Abeer Khalaf and Gloria A. Moore

Chapter 6

Evaluation of Factors Affecting European Plum
(Prunus domestica L.) Genetic Transformation 123
Yuan Song, Fatih Ali Canli, Farida Meerja, Xinhua Wang,
Hugh A. L. Henry, Lizhe An and Lining Tian

Chapter 7

Genetic Transformation of Wheat:
Advances in the Transformation Method and Applications
for Obtaining Lines with Improved Bread-Making Quality
and Low Toxicity in Relation to Celiac Disease 135
Javier Gil-Humanes, Carmen Victoria Ozuna, Santiago Marín,
Elena León, Francisco Barro and Fernando Pistón

81

VI

Contents

Chapter 8

Maize Transformation to Obtain Plants
Tolerant to Viruses by RNAi Technology 151
Newton Portilho Carneiro and Andréa Almeida Carneiro

Chapter 9

Genetic Transformation
of Triticeae Cereals for Molecular Farming 171
Goetz Hensel

Chapter 10

Genetic Transformation of Forest Trees 191
Osvaldo A. Castellanos-Hernández, Araceli Rodríguez-Sahagún, Gustavo J. Acevedo-Hernández and Luis R. Herrera-Estrella

Chapter 11

Agrobacterium-Mediated Transformation
of Indonesian Orchids for Micropropagation 215
Endang Semiarti, Ari Indrianto, Aziz Purwantoro,
Yasunori Machida and Chiyoko Machida

Chapter 12

Transient Transformation of Red Algal Cells:
Breakthrough Toward Genetic Transformation
of Marine Crop Porphyra Species 241
Koji Mikami, Ryo Hirata, Megumu Takahashi,...
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