Gel Electrophoresis

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Alexander Davis

Period: 1 Group Partners: Jenny Case

Blake Rebek Matthew Heigm Christian Hogdohm

Gel Electrophoresis

I. Introduction:
A typical electrophoresis has five major parts: the electrical current, DNA, RNA, or protein sample, a support medium, the liquid electrical conduction medium and the detecting system or stain. DNA fragments are put into the wells at an end of the gel slab. An electric current is at both parts of electrophoresis chamber to create an electric field within the gel. DNA fragments migrate within this gel depending on the size. The separated molecules form bands from one end of the gel to the other. Each band represents a specific fragment of DNA. Electrophoresis can be used for many health issues, such as identifying diseased tissue, genetic dysfunctions, pinpoint cancer types and read the nucleotide base sequence of a particular gene.

II. Problem:
Can we use gel electrophoresis to find the molecular weight of the sample

dyes?

III. Hypothesis:
I believe that the electrophoresis gels will resolve, or separate on the gel

according to their molecular size. The dyes are negatively charged and

will go to the positive part of the E Gel.

IV. Procedure/Material:
• E-Gel Apparatus
• Plastic container of dyes
• Beaker of micro-pipette tips
• Micropipettes
• Wrapped gel

1. The power base was plugged into...
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