Frog Leg Lab Report

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The experiment was conducted by using frog’s legs to understand how the muscle response to different levels of stimulations, and the levels of the twitch is also related to the number of motor units recruited. We used the program LabScribe to collect the data from the graph, and we also used the stimulator to stimulate the muscle through computer. We found out when the response reached the threshold and plateau. For the second experiment, we increased the Frequency from 0.5 Hz to 1Hz and then 2 Hz, 4Hz, 8Hz, 16Hz, 32Hz, 64Hz, and we found out when the graph reach to the summation and tetanus. Third, we used the graph in tetanus to calculate the speed of contraction and relaxation. Fourth, we rotated the femur clamp to adjust the muscle length and looked at the changed in response in volts, and found out the optimal length. In result, we found out the threshold voltage is about 0.2 volts and the plateau is at 2.2 volts, the summation started at 16Hz, and it reached tetanus at 64Hz, for the optimal length, we did not get enough time to finish it, and it should be somewhere higher then 35 mm. Introduction: Introduction:

First, we needed to prepare the frog by remove the skin from the leg and cut off the muscle on the femur, and find the gastrocnemius muscle, which is the big muscle on the lower leg, and then we found the tendon of Achilles that is below the heel, and cut it at the lowest point of the tendon, and then cut off the tibia bone. Moreover, we measured the resting length of the muscle. After that, we tie a suture around the tendon and placed it on the femur clamp, and then we placed the stimulating electrodes touched the belly of the muscle, and the stimulating electrodes was attached into the stimulator output panel. We also needed to keep the muscle moist all the time by using frog ringers solution.

Next, we started the first experiment by using the computer and open the program called LabScribe. This program allowed us to...
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