Faculty of Pharmacy
Organic Chemistry Laboratory
APPLICATION OF DIFFERENT KINDS OF TEST TO CLASSIFY HYROXY- AND CARBONYL-CONTAINING COMPOUNDS
Jane Catherine SP. Villanueva, Edenn Claudine C. Villaraza, Lorenz Oliver C. Villegas and Cristel Bernice T. Wee
Group 10 2G-Medical Technology Organic Chemistry Laboratory
Hydroxyl group refers to a functional group containing OH- when it is a substituent in an organic compound. It is also known as the characteristic functional group of alcohols and phenols. On the other hand, carbonyl group refers to a divalent chemical unit consisting of a carbon and an oxygen atom connected by a double bond. It is known as the characteristic functional group of aldehydes and ketones instead.
In this experiment, hydroxyl- or carbonyl- containing samples were given to the group for examination. The samples were analyzed through different tests namely the involvement of the solubility of alcohols in water, the Lucas Test, the Chromic Acid Test or also known as Jones Oxidation, the 2,4-Dinitrophenylhydrazone (2,4-DNP) Test, the Fehling’s Test, the Tollens’ Silver Mirror Test, and the Iodoform Test. The solubility of alcohols in water test showed that the sample, benzyl alcohol was immiscible while ethanol was the most miscible from all the other compounds used. While in Lucas Test which was used to differentiated the primary, secondary, and tertiary alcohols had turned tert-butyl alcohol into a cloudy solution afterwards. In Chromic Acid Test which was a test for oxidizable compounds or any compounds that possess reducing property would yield to a blue green solution if it reacted positively. This was seen in all the sample used in this test except for acetone. Whereas Dinitrophenylhydrazone (2,4-DNP) Test was preformed to test for aldehydes and ketones which would result to a yellow orange precipitate if it was positively reacted. All the compounds subjected to this test namely... [continues]
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