Formal Lab Report Plant Bio
Formal lab report:
Abstarct:
The Purpose of this experiment was to perform Agrobacterium-mediated transformation in Wild type Arabidopsis thaliana Columbia by using somatic plant transformation method. The whole process lasted for over a period of 11 weeks and we were successful in getting transformed plants. Agrobacterium tumefaciens strain containing pMP90 (Ti-helper) plasmid and pCAMBIA1391 (T-DNA) plasmid was used for this plant transformation. pCAMBIA1391 plasmid was constructed by cloning Brassica rapa seed specific Napin promoter to control expression of GUS (beta-glucuronidase) reporter gene. Leaves were cut in small portions and were transferred to media containing antibiotic (Kanamycin), making the media selective for explants containing antibiotic resistant genes. The growth of shoots from the explants then grew and confirmed for the successful transformation of our gene of interest.
Introduction:
Plant transformation has its roots in the research on Agrobacterium that was being undertaken in the early 1980s. Advances in the technology have been due to development of a range of Agrobacterium-mediated and direct DNA delivery techniques, along with appropriate tissue culture techniques for regenerating whole plants from plant cells or tissues in a large number of species Agrobacterium-mediated genetic transformation is the dominant technology used for the production of genetically modified transgenic plants. Agrobacterium tumefaciens is a naturally occurring gram negative bacterium which causes crown gall disease in over 140 species of dicot plants. The strains used for transforming Arabidopsis contain T-DNA in the Ti plasmid which are then introduced into the plant cell. This Ti plasmid releases the T-DNA once in the plant cell which gets incorporated into a semi-random location in the plant genome. Once incorporated, T-DNA induces the plant to produce hormones such as auxin and cytokinin which are required for Agrobacterium nutrition. The gene of
Abstarct:
The Purpose of this experiment was to perform Agrobacterium-mediated transformation in Wild type Arabidopsis thaliana Columbia by using somatic plant transformation method. The whole process lasted for over a period of 11 weeks and we were successful in getting transformed plants. Agrobacterium tumefaciens strain containing pMP90 (Ti-helper) plasmid and pCAMBIA1391 (T-DNA) plasmid was used for this plant transformation. pCAMBIA1391 plasmid was constructed by cloning Brassica rapa seed specific Napin promoter to control expression of GUS (beta-glucuronidase) reporter gene. Leaves were cut in small portions and were transferred to media containing antibiotic (Kanamycin), making the media selective for explants containing antibiotic resistant genes. The growth of shoots from the explants then grew and confirmed for the successful transformation of our gene of interest.
Introduction:
Plant transformation has its roots in the research on Agrobacterium that was being undertaken in the early 1980s. Advances in the technology have been due to development of a range of Agrobacterium-mediated and direct DNA delivery techniques, along with appropriate tissue culture techniques for regenerating whole plants from plant cells or tissues in a large number of species Agrobacterium-mediated genetic transformation is the dominant technology used for the production of genetically modified transgenic plants. Agrobacterium tumefaciens is a naturally occurring gram negative bacterium which causes crown gall disease in over 140 species of dicot plants. The strains used for transforming Arabidopsis contain T-DNA in the Ti plasmid which are then introduced into the plant cell. This Ti plasmid releases the T-DNA once in the plant cell which gets incorporated into a semi-random location in the plant genome. Once incorporated, T-DNA induces the plant to produce hormones such as auxin and cytokinin which are required for Agrobacterium nutrition. The gene of