Biology Period, 1st Ms. Robinson
Juanita High School
December 13, 2011
Formal Lab Report
This lab addresses how people’s metabolism directly affects their performance when faced with a situation that involves exercise for one, and two minutes. Using and understanding Cellular Respiration is something you will need to learn before you can fully grasp this Formal (Lee, Susan. Bio Home. N.p., 15 Feb. 2005. Web. 9 Dec. 2011. <http://biology.clc.uc.edu/courses/bio104/cellresp.htm>.) Lab Report. First of all, you will need to know the equation for Cellular Respiration; Drayton, (Josh D. About Biology. About.com, 29 June 2007. Web. 7 Dec. 2011. <http://biology.about.com/od/cellularprocesses/a/cellrespiration.htm>.) C2H12O6 + 6 H2O --- 6 CO2 + 6 H2O + 36 ATP (Energy)
There are three total steps that Cellular Respiration goes through. The first step macromolecules into simple subunits, the second step is the breakdown of those newly made subunits. The second step changes those subunits into acetyl CoA, this is needed help make ATP and NADH. Step three is the final step, witch turn CoA into CO2 this is almost a short cut from electronic transport. This is the equation for Cellular Respiration, basically all this means is that it requires energy to create energy. So what the lab does, is determines how much energy it takes from person to person to exercise and how CO2 production, Breathing Rate, and Pulse Rate fluctuate. In America we are so concerned about looks and fitness, but if this is true than why is there such a problem with obesity. Well, if you read, and understand what has been done in this lab, than you will be able to adjust your metabolism so as to help decrease, (or increase) your overall weight. This lab does not help you change your metabolism, but shows the current average of the class. By conducting this experiment, I believe that the obvious with show. That if you exercise for 1 minute and then 2 minutes than your breathing rate, pulse rate, and Co2 production rate will increase because of the amount of time working out also increases. (My journal is this citation)
* Beaker/ Test Tube/ Cup
* Bromthymol Blue Solution
* Stop Watch
PART A: RESTING (NO EXERCISE) Measuring Carbon Dioxide production 1. Measure 20mL of tap water and pour into a small beaker 2. Using a dropper, add 8 drops of bromthymol blue (BTB) to the water in the beaker 3. Using a straw, CAREFULLY exhale into the BTB solution (CAUTION: Do not inhale the solution. Breathe in through your nose and out through your mouth) 4. Time how long it takes for the blue solution to turn yellow. Record the time in TABLE 1. 5. Wash out the beaker and repeat steps 1-4 two more times. Record data in TABLE 1 6. Average the results of the 3 trials. Record average in TABLE 1 7. Report your averages to the Class Data Table 1
Measuring Breathing Rate:
1. Count the number of breaths (1 breath = inhale + exhale) you take in 1 minute. Record this in TABLE 2 2. Repeat this 2 more times.
3. Average the 3 trials to get your average breathing rate. Record this in Table 2. 4. Report your averages to the Class Data Table 2
Measuring Heart Rate:
1. Have your partner take your pulse
2. Count the number of heart beats in 30 seconds and multiply that number by two. Record this in TABLE 3. 3. Repeat this 2 more times.
4. Average the 3 trials to get your average heart rate. Record in TABLE 3. 5. Report your averages to the Class Data Table 3
PART B: INCREASED MUSCLE ACTIVITY (EXERCISE!!!)
1. Exercise for exactly 1 minute by doing vigorous activity - jumping jacks or running or push-ups (be sure to note which type of exercise you do in your data) 2. After 1 minute of exercise, immediately exhale CAREFULLY through the straw into the BTB solution (breathe in through your nose and out through your mouth)....
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