Finance and Banking

Topics: Communication, Communication noise, Green fluorescent protein Pages: 3 (733 words) Published: May 13, 2013
“Identify an experience in which you failed to communicate a message.” As mentioned in the communication process, communication has only succeeded when the information given by the sender has been received and understood by the recipient. If the recipient has not understood the information, then this may not necessarily be the recipient's fault. Typically, ineffective communications can be attributed to one of three things: 1. A poor message;

* The message was too short;
* The message was too long;
* The message was ambiguous
2. Poor transmission;
* That the message is being delivered in a wrong format that the recipient both does not expect and understands; * That the message is being delivered when the recipient does not need it, and where the recipient will not expect to find it. 3. Poor reception;

* A lack of awareness;
* Obstructionism;
* A lack of understanding;

A striking example where I failed to communicate a message was my first day as an ‘A’-Level biology teacher at a certain private college. I was lost and found myself tutoring a form 4 class instead of a form 6 class. Some of the teaching staff read, “Human monocytes were cultured for 24 h in serum-free AIM-V medium, followed by 24-h maturation by polyriboinosinic polyribocytidylic acid (polyI:C). Short term cultured, polyI:C-maturated DC, far more than immature DC, showed typical mature DC markers and high allogeneic stimulatory capacity and had high autologous stimulatory capacity in an influenza model system using peptide-pulsed DC. Electroporation of mRNA as an Ag-loading strategy in these cells was optimized using mRNA encoding the enhanced green fluorescent protein (EGFP). Monocytes electroporated with EGFP mRNA, followed by short term, serum-free differentiation to mature DC, had a phenotype of DC, and all showed positive EGFP fluorescence. Influenza matrix protein mRNA-electroporated monocytes cultured serum-free and maturated with...
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