DEPARTMENT OF BIOTECHNOLOGY
KULLIYYAH OF SCIENCE
ANALYTICAL METHODS FOR FUNCTIONAL FOODS & NUTRACEUTICALS
FAT & OIL FROM OIL-SEEDS: PART III
ASSOC. DR. JAMALUDDIN BIN MOHD DAUD
ABDUL AZIM ADNAN
ABDUL AZIM BIN ADNAN
In gas chromatography, gaseous mobile phase or sometime called carrier gas is used to transport the gaseous analyte (compound or sample) through the column. In gas liquid partition chromatography, the nonvolatile liquid which is the stationary phase bonded to the inside of the column or to a fine solid support. In gas-solid Adsorption chromatography, analyte is adsorbed directly on solid particles of stationary phase. In the schematic diagram of gas chromatography;
The volatile liquid sample is injected through a septum (a rubber disk) into a heated port, in which it rapidly evaporates. Vapor than swept through the column by He, N2, or H2 carrier gas. The separated analytes flow through a detector, whose response is displayed on a computer. The column should be hot enough to provide sufficient vapor pressure for analytes to be eluted in a reasonable time. The detector is preserved at a higher temperature than the column so analytes will be gaseous.
1. To learn and understand the operating system in gas chromatography 2. To detect the organic compound present in the sample
3. To compare the peak of sample with the peak from the external standard
APPARATUS AND MATERIAL
Vials of sample (acetone, methanol and unknown)
1. The principle and handling of gas chromatography were demonstrated by lab instructor 2. Temperature, gas carrier and gas flow rate was set up in suitable condition 3. The three samples were prepared and the sample was run one after another 4. Data is obtained from the detector and was...