Factors That Influence Catalase Activity

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Factors That Influence Catalase Activity
Sumeet Salvi11/16/12
AP BiologyBlock 3

Purpose:
The purpose of this experiment was to test and observe the numerous amounts of factors that influence the activity of the enzyme and that can be done by releasing oxygen gas in a fixed condition and change conditions one at a time. Hypothesis:

If a protein is acquainted with a disadvantageous condition that could denature the protein, then it will not release as much Oxygen gas as it normally should. Results/Data:
Part A: The Time Course of Enzyme Activity
Time (30 Second Intervals)| Gas Levels |
0:30| 76|
1:00| 77|
1:30| 79|
2:00| 82|
2:30| 90|
3:00| 94|
3:30| 96|
4:00| 98|
4:30| 99|
5:00| 102|

Part B: The Effect of Enzyme Concentration on Enzyme Activity Time (30 Second Interval)| 75% Enzyme Concentration | 50% Enzyme Concentration| 25% Enzyme Concentration| 0:30| 83| 88| 72|
1:00| 84| 88| 73|
1:30| 84| 89| 74|
2:00| 85| 90| 75|
2:30| 86| 91| 76|
3:00| 86| 92| 76|
3:30| 87| 92| 76|
4:00| 87| 93| 77|
4:30| 88| 94| 77|
5:00| 89| 94| 78|

Part C: The Effect of Temperature on Enzyme Activity
Time (30 Second Intervals)| 5oC| 37oC| 100oC|
0:30| 56| 11| 0|
1:00| 57| 12| 0|
1:30| 58| 18| 0|
2:00| 59| 24| 0|
2:30| 59| 31| 0|
3:00| 59| 40| .25|
3:30| 59| 48| .25|
4:00| 60| 55| .25|
4:30| 60| 62| .3|
5:00| 60| 67| 3|

Part D: The Effect of pH on Enzyme Activity
Time (30 Second Intervals)| pH 4 solution| pH 7 solution| pH 10 solution| 0:30| 7| 9| 10|
1:00| 12| 16| 18|
1:30| 17| 20| 23|
2:00| 20| 23| 28|
2:30| 23| 25| 32|
3:00| 26| 28| 34|
3:30| 28| 30| 36|
4:00| 30| 33| 38|
4:30| 33| 35| 39|
5:00| 34| 36| 40|

Part E:
Time (30 second intervals)| 0% Substrate Concentration| 0.3% Substrate Concentration| 1.5% Substrate Concentration| 3% Substrate Concentration| 0:30| 0| 6| 8| 76|
1:00| 0| 10| 16| 77|
1:30| 0| 16| 24| 79|
2:00| 0| 20| 29| 82|
2:30| 0| 24| 35| 90|
3:00| 0| 28| 38| 94|
3:30| 0| 30| 43| 96|
4:00| 0| 32| 47| 98|
4:30| 0| 34| 50| 99|
5:00| 0| 37| 54| 100|

Part F: The Effect of Ionic Concentration on Enzyme Activity Time (30 Second Intervals)| 10% NaCl Concentration| 2% NaCl Concentration| 0% NaCl Concentration| 0:30| 3| 9| 8.5|
1:00| 4| 14| 13.5|
1:30| 6| 17| 18|
2:00| 7| 20| 21|
2:30| 8| 23.5| 25|
3:00| 9| 26| 28|
3:30| 9.5| 28| 31|
4:00| 10| 31| 33|
4:30| 11| 33| 35|
5:00| 11.5| 34| 37|

Conclusion:
The data from the lab shows if enzyme concentration, temperature, pH, Substrate Concentration, or ionic concentration is altered, then the enzyme will not have as much of an effect on the substrate as it should. Analysis:

The main focus of this lab is the enzymes. Enzymes are proteins that catalyze chemical reactions of other substances without altering the shape of it. Enzymes are very important to humans because the substrate needs to attach to the active site in order to speed up chemical reactions. They catalyze chemical reactions lowering the activation energy because it can allow chemical reactions to occur in colder temperatures. In this lab, the enzyme was the yeast catalase. This particular enzyme eliminates Hydrogen Peroxide and converts it into Oxygen and water. Enzyme concentration is the amount of enzymes you have in a particular solution. pH level is a scale that measures the acidity and alkalinity of the substance based on the amount of Hydroniums ions it has in it. Substrate concentration is the amount of substrates in a particular solution. Ion Concentration is the amount of ions in a particular solution.

In Part A of the lab, the catalase was reacting the Hydrogen Peroxide to create Oxygen...
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