Factors That Affect Enzyme Reaction Rate

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Lab Report
Factors That Affect Enzymes Reaction Rate

Name of lab:
Effects of temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity

Introduction:
Enzymes are the most important types of proteins, they act as catalysis (speed up chemical reactions). If enzymes didn’t exist, biochemical reactions would act to slowly and they couldn’t keep up with the metabolic functions. Enzymes have a three-dimensional structure that is really complex. This structure consists of one or more polypeptide chains, they form an active site, which is an area in which the substrate eventually will fit. The four factors that affect the activity and reaction rate of an enzyme are temperature, pH, enzyme concentration and substrate concentration.

Research Question/Hypothesis:
The hypothesis is that when the temperature is higher than 40⁰ C, the enzyme catalysis will increase. That the enzyme activity with the pH effect is that if it isn’t at the optimum pH value there will be a total loss of the enzyme activity. As the change in enzyme concentration increases the activity rate will increase if there is a proportional amount of enzyme concentration and substrate.

Variables:
Independent: pH, enzyme concentration, substrate concentration and enzymatic activity. Dependent: the reaction rate
Control variable: temperature and amount of substrates and enzymes added.

Materials:
Phosphate
Buffers
Beaker
Catechol
Potato Juice
Parafilm
Test Tubes

Procedure:
To study the effect of temperature:
1.Three different test tubes where filled with 3mL of phosphate. 2.They were set in three different temperature settings. First tube was placed in an ice-water bath for ten minutes, the second in a room temperature until 21⁰C was reached, and the third tube was placed in a beaker of warm water. To study the effect of pH:

1.The pH values of pH 4, pH 6, pH 7, pH 8, and pH 10 was used to fill five separate test tubes with 3 mL each. 2.To...
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