Experiment to Show the Effect of Temperatutre on Enzyme Activity

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  • Topic: Rachel, Cassie, Marco
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  • Published : January 10, 2013
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EXPERIMENT TO SHOW THE EFFECT OF TEMPERATURE ON ENZYME ACTIVITY

Table of raw data of experiment to show effect of temperature on enzyme activity Temperature (ᵒc) (+/-0.05)| Time taken for pink solution to turn back to white (s) (+/- 0.05)| | Repeat1| Repeat2| Repeat3| Repeat4| Repeat5|

21 (Room Temperature)| 414.0| 286.0| 286.0| 297.0| 294.0| 40| 122.0| 88.0| 125.0| 86.0| 92.0|
60| 176.0| 162.0| 161.0| 166.0| 180.0|
80| 356.0| 367.0| 396.0| 85.0| 438.0|
100| Greater than 10| Greater than 10| Greater than 10| Greater than 10| Greater than 10| During the experiment I noticed that at room temperature the solution turned from pink to white quite slow but then as the temperature increased it turned faster. The time taken for the solution gradually decreased, at one point it started increasing, then eventually the solution did not turn white at all at 100ᵒC. During the experiment I noticed that at room temperature the solution turned from pink to white quite slow but then as the temperature increased it turned faster. The time taken for the solution gradually decreased, at one point it started increasing, then eventually the solution did not turn white at all at 100ᵒC.

Table of processed data of experiment to show the effect of temperature on enzyme activity

Graph of average time taken(s) against temperature(ᵒC)

Conclusion of experiment to show the effect of temperature on enzyme activity Temperature has an effect on enzyme activity. As temperature increases, enzyme activity increases making it work faster. The enzyme activity increases till an optimum temperature is reached, from then the activity of the enzyme decreases as further increase in temperature starts to denature the enzyme. Enzyme activity will eventually stop when it gets completely denatured. This is evident in this experiment, at room temperature (21ᵒC) the enzyme was able to break down the fat in the milk (turning the solution...
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