Experiment on Osmosis

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GCSE - Human Physiology and Health

An experiment to investigate what affect sucrose solution has on potato tissue.


Osmosis is the movement of water molecules, across a partially permeable membrane from a region of high concentration of water to a low concentration of water molecules. A partially permeable membrane is a membrane with holes in it small enough for only water to go through it. Big molecules like glucose cannot fit through it. Osmosis allows plants to take in water through their roots, and is also used in kidney dialysis machines to filter the blood.


The aim of this experiment is to investigate how potato tissue changes when placed in sucrose solution of different concentrations. I want to find out if and how osmosis differs, when the concentration is changed.

The Fixed Variables
There are several fixed variables I can control in order to get a different outcome to my experiment: 1)Temperature of the atmosphere in which the investigation takes place 2)Surface on which the investigation takes place

3)Length of time in which the chip is submerged in the solution 4)Position of potato chip
5)Size of potato chip
6)Amount of solution
7)Concentration of solution.

1,2,3&4) gather all the equipment needed, as per the list below. A range of sucrose solutions will then be prepared with concentrations of 0.00 molar, 0.25 molar, 0.50 molar, 0.75 molar and 1.00 molar. You will need to measure 15ml of each of the solutions in a measuring cylinder. You can check that the volume is at the correct measurement, by bending down to eye level. This will give you an accurate reading. You can also alter the concentration by using a pipette. You will then place each 15ml of solution into the correct specimen tubes. You then need to add a label to each specimen tube to advise what sucrose solution is in what. For organisation’s sake, we set up all of the specimen tubes at once, and labelled them individually to...
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