Experiments to investigate the effect of enzyme concentration of the activity of the enzyme catalyse. This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalyses. Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes are also proteins that are folded into complex shapes that allow smaller molecules to fit into them. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalyse is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from the cells. Hydrogen Peroxide is the poisonous by product of metabolism. Catalyse speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below. Two examples of reactions that produce H2O2 are conversions of amino acids into fuel molecules and conversion of lipids to carbohydrates. It can damage DNA, protein and lipid membranes and may even be a causative factor in cancer. There are some human immune system cells that actually use H2O2 to kill foreign invaders.
Hydrogen Peroxide---------->Water + Oxygen, 2H2O2-------------->2H2O+O2
The activity of the enzyme can be measured by collecting the oxygen produced over which is called period of time. Enzyme activity will be measured as a function of temperature. A protocol for measuring activity as a function of enzyme concentration is also provided for those that have access to a 1 mL adjustable pipette. They can be denatured by heat, and can easily be broken down by proteases when cells are homogenized. To preserve activity of proteins in solution, it is important to keep the...
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