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Meera Patel
Enzyme lab

Assignment 1 :- Effect of Temperature on Invertase Activity, Determining the Starting Velocity. Enzyme is the fastest known catalyst. They increase the velocity and rate of reactions in living cells. Diverse range of proteins works as enzyme with their specific temperature point, pH etc. Enzymes react with the molecules known as substrates. Binding site of an enzyme and a substrate is known as active site. The amount of enzyme present in a reaction is measured by the activity it catalyzes. The relationship between activity and concentration is affected by many factors such as temperature, pH, etc. Though enzymes are important for biological reactions, certain factors play an important role in the activity. Enzymes works at their optimum temperatures and pH condition, that means the point where the activity is highest. If the factor goes above the optimum values, proteins get denatured due to the alterations in the bonds like hydrogen bonds and Van Der Waals attractions. Enzyme kinetics is greatly influenced by the substrate concentration and velocity. The enzymatic catalyzing begins by the process of binding of an enzyme to a substrate and forming the enzyme substrate complex called ES. These types of reactions are either reversible or irreversible. Hypothesis: The enzyme becomes denatured at high temperatures. That means that the chemical bond holding the structure together break down, so the shape of the enzyme changes and can no longer operate as a 'key' to trigger the reaction. In terms of lower temps, then there is insufficient energy of activation to push the reaction forward.

What is the optimal temperature for invertase activity? Is this what you expected? Would invertase isolated from any two organisms (for example, yeast invertase vs. invertase from the small intestine of humans) show the same optimum temperature? Why or why not? Explain your answers. The optimal temperature for the invertase activity is 50...
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