Enzymes!!

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Enzyme Catalysis Lab

Problem:
Before the lab, one should understand:
The general functions and activities of enzymes;
The relationship between the structure and function of enzymes •The concept of initial reaction rates of enzymes;
How the concept of free energy relates to enzyme activity; •That change in temperature, pH, enzyme concentration, and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and •Catalyst, catalysis, and catalase

After the lab, one should be able to:
Measure the effects of changes in temperature, pH, enzyme concentration, and substrate concentration on reaction rates of an enzyme-catalyzed reaction in a controlled experiment; and •Explain how environmental factors affect the rate of enzyme-catalyzed reactions.

Hypothesis:
When one mixes catalase and hydrogen peroxide with sulfuric acid for a short period of time, there is a high rate of increase for the decomposition of hydrogen peroxide to hydrogen and water. However, after one mixes these substances together for a longer period of time than necessary, substrate becomes overly-saturated in the solution which causes the enzyme not to work properly.

Materials:

Goggles
Reaction vessels (cups)
Gloves
Syringes
Plastic Wrap
Rubber Bands
Potassium Permanganate
Sulfuric Acid
Catalase Extract
Hydrogen Peroxide

Procedure:
First, we made the base line assay to determine the fluctuation from the normal reaction mixture without catalase. This was the index of the initial concentration of H2O2 in solution. To establish a baseline, start by putting 10 mL of 1.5% H2O2 into a clean glass beaker. Add 1 mL of H2O (instead of enzyme solution). Add 10 mL of H2 SO4 (1.0 M). Mix well. Remove a 5-mL sample. Place this 5-mL sample into another beaker and assay for the amount of H2O2 as follows. Use a syringe to add KMnO4 , a drop at a time, to the solution until a persistent pink or brown color is...
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