The purpose of this lab is to observe through experimental procedure on how enzymes affect chemical reactions.
An enzyme is a protein that serves as a biological catalyst. A catalyst is any substance that increases the rate of a chemical reaction, (done by lowering the activation energy of the reaction). In this experiment we are using Hydrogen peroxide (the substrate for this experiment). Peroxidase is a soluble enzyme normally found in the cytoplasm of cells. Our Experimental design was to find out if decreasing the amount of substrate will affect the reaction rate of the enzyme. For this experiment we used yeast as our peroxidase. The amount of enzyme was kept constant for this experiment. Because the catalyst remained constant the group’s original hypothesis was, as we decrease the amount of substrate reaction will speed up. (The slope of the line will get steeper.) We decreased the amount of substrate for each reaction assuming that the result would increase the reaction rate of the reaction. Vocabulary:
Activators Inhibitors Substrate Catalyst Optmum Temperature Materials:
Logger Pro and Laptop computer
Using a graduated cylinder (to measure accurately) pour the desired amount of substrate and water into each test tube. Do not mix the enzyme with the mixture until you are ready to measure the rate of reaction for each individual tube. The 1’s are for the first trial and the 2’s are for the second trial. (If time permits, perform the experiment at least two times.) 1. Pour 5ml of substrate, 0ml of enzyme (no enzyme for negative control), and .5ml of water into test tube A1. 2. Seal test tube A1 with a stopper immediately after the enzyme is added. 3. Record the reaction rate using the Logger Pro software. 4. Repeat steps 1-3 using the new measurements
Substrate Enzyme Water...