Enzyme Lab Write Up

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AP Biology
Sunday, October 24, 2010 Enzyme Lab Write-Up

To observe how an enzyme affect the speed of chemical reaction.
To describe how the concentration of an enzyme affects its ability to work.

Depending on the concentration of the catalase which the disk is soaked in, it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas.

Since the rate of reaction can be lowered by the addition of catalysis such as an enzyme. Moreover to increase the effectiveness of an enzyme one must add additional amount of enzyme due to saturation. Therefore the 100% will have the greatest effect because is has the highest concentration of the catalase.

Independent variable:
The independent variable is the Potato extract.

Dependent variable:
The dependent variable is hydrogen peroxide.

Controlled variable:
Amount of hydrogen peroxide
Potato extract
Size of paper disk
Temperature of potato extract

Level of treatment:
Our level of treatment for the independent variable is based in 20 intervals. 20%, 40%, 60%, 80%, 100%

2000 ml of potato extract
100 ml graduated cylinder
3% H2O2 solution
Marking pen
Filter paper disk
1000 ml of H20
8 - 100 ml beakers
Hot plate
Label six beakers in intervals of 20%
Obtain catalase solution from your teacher and dilute according to chart on lab Label another 100 ml beaker 3% H2O2. Label the 50 ml ‘reaction chamber’ Using forceps, carefully dip one disk into a beaker

Hold in liquid for about three-seconds and then touch the disk to the inside of the beaker to remove excess drops of liquid. Place the filer paper disk on the bottom of the beaker which contains hydrogen peroxide and release it saying now to your timekeeper. Record how long it takes for the disk to rise to the surface.


The catalysis solution is stored on ice...
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