Enzyme Lab

Topics: PH, Enzyme, Catalase Pages: 4 (938 words) Published: October 22, 2008
Enzyme Activity Lab

Hypothesis:

1] Are enzymes reusable?

Yes, enzymes are reusable.

2] Which of the following do you think contain catalase? [Manganese dioxide, chicken meat, apple, carrot, potato, or chicken liver]

I think that the manganese dioxide, apple, and carrot contain the catalase.

3] Would grinding the materials affect enzyme activity?

I think that grinding the materials will increase enzyme activity.

4] Would temperature affect enzyme activity?

I think that at normal body temperature, regular enzyme activity will occur.

5] What about pH levels?

I think that at a balanced pH level (pH 7) like water, enzyme activity will be at the highest.

Objectives:

-To demonstrate the activity of an enzyme in living tissues

-To learn how changes in temperature and pH affect the activity of a catalase

-To experimentally test for the presence of catalase in living tissue

-To analyze factors affecting enzyme activity

-To determine the affect of particle size on enzyme activity

Materials:

3% Hydrogen PeroxideScissors

Straight-edge razor blade10 Test Tubes

Mortar and PestleBlender/Food Processor

Test Tube RackDistilled Water
PipetteGoggles
Manganese DioxideStirring Rod
Fresh Chicken MeatForceps
Fresh AppleThermometer
Fresh CarrotIce bath
Hot PlateFresh chicken liver

Conclusion:

In this lab, we were able to observe the affect that catalase has on different substances such as pieces of liver, chicken, apple, potato, and carrot. In the experiment, if the substance started to create bubbles, then it contained the catalase that could break down the hydrogen peroxide or H2O2.

The reaction between liver and hydrogen peroxide or H2O2 was an exothermic reaction. After having reacted, the liver and hydrogen peroxide or H2O2 mixture caused the temperature to rise. When heat is being released from a reaction it is caused exothermic. In the body hydrogen peroxide or H2O2 is...
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