Enzyme Lab

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Introduction

Enzymes are proteins produced by living organisms to speed up the rate in which chemical reactions occur. This process can happen fast, slowly, or stop the chemical reaction all together depending on the temperature, pH and concentration. Catalase is one of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However, when catalase is used it breaks it down into hydrogen and oxygen, which is much safer to have in the body.

A substrate is the material that is being broken down. In this case, the substrate would be hydrogen peroxide. The catalase, located in the liver, is being used to break down the substrate.

Problem

The purpose of this lab is to see if the enzyme, catalase, can be manipulated. We are testing the effects of pH, temperature and enzyme concentration.

Hypothesis
Controlled
When we add peroxide to the liver it will bubble a lot and break down faster.
When we add peroxide to the liver it will not bubble a lot and it will not break down faster. pH
When acid is added to the liver, it will increase the reaction rate and a large amount of bubbles will surface.
When acid is added to the liver, it will not increase the reaction rate and a large amount of bubbles will not surface.
When water is added to the liver, there will be no significant change to the liver.
When water is added to the liver, there will be a significant change to the liver.
When salt water is added to the liver, there will be a slight increase in rate of reaction and a small amount of bubbles will surface.
When salt water is added to the liver, there will not be a slight increase in rate of reaction and a small amount of bubbles will not surface. Temperature
When the liver is placed in the boiling water, then peroxide is added, it will bubble a little and change color.
When the liver is placed in the...
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