Lab Report: 10
This test is often used to see if you have been exposed to viruses or other infectious substances. It is frequently used to screen for present or past infections.
ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An ELISA or enzyme-linked immunosorbent assay is a method used in the laboratory to aid in the diagnosis of a wide range of diseases. This test is performed on blood or urine and is used for measuring the amount of a particular protein or substance in these bodily fluids, such as infectious agents, allergens, hormones or drugs. This test relies on the interaction between components of the immune system called antigens and antibodies. Antibodies are proteins produced by the body to identify and neutralize any foreign substances that may be encountered, such as viruses and bacteria. The substances to which antibodies are produced are known as the antigens as they stimulate an immune response. If antibodies are being detected for example to HIV, then a portion of the HIV virus is attached to a solid surface such as a tube or plate. This will act as the antigen. Your serum will then be added to the tube and if it contains antibodies to the antigen then it will bind to it. Another antibody which recognizes the HIV antibodies is then added and binds to any bound antibody. This second antibody is linked with a chemical known as an enzyme (an enzyme speeds up a chemical reaction) and in the final step a substance (peroxidase) which reacts with the enzyme on the antibody is added to produce a colored product. If the test is positive then a color reaction will occur. If you don’t have antibodies to that certain antigen then no reaction will occur and no color change will be seen. Materials:
PBS, HIV antigen, Peridase, donor 1, donor 2, well, tips, pipette, 2C AB
First you label well...