Effects of Photosynthesis of Spinach Leaves in Different Light Conditions
Photosynthesis is the process of converting solar energy, carbon dioxide (CO2), and water (H2O) into carbohydrates (CH2O) and oxygen (O2). Sometimes the end product of photosynthesis is glucose (C6H12O6). Photosynthesis takes place in the chloroplasts of plant cells. Here membranous thylakoids are stacked in grana surrounded by the stroma. During the light reactions, pigments within the thylakoid membranes absorb solar energy, water is split, and oxygen is released. (Mader, 2010) In this study, we are interested in examining the effects of photosynthesis in spinach leaves in the presence of different light conditions. The light conditions that will be tested are no light, white light and green light. Therefore, we hypothesized that the O2 rate of consumption would occur more in the green light condition and CO2 rate of consumption would occur more in the no light condition. Methods
A laptop computer with Logger Pro Computer Program connected to a Vernier LabQuest Computer Interface. A Vernier O2 Gas Sensor and a Vernier CO2 Gas sensor connected to the Vernier LabQuest Computer Interface are used to measure the amount of oxygen and carbon dioxide produced. The spinach leaves are placed in the 2000 mL respiration chamber. A 150 Watt goose neck lamp is used as the primary light source. Aluminum foil is wrapped around the 2000 mL respiration chamber is used to test the no light condition. The 500 mL Beaker is filled with water and placed in between the respiration chamber and the goose neck lamp. The instructions on the photosynthesis lab handout were used to carry out the experiment with the following change. Green food coloring was added to the 500 mL Beaker to test the effects of photosynthesis in green light. Results
After collecting data for 15 minutes for spinach leaves in each light condition, we found that the rate of CO2 consumption for the spinach leaves were higher...
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