Effects of long-term ketamine administration on rat bladder protein levels: A proteomic investigation using two-dimensional differece gel electrophoresi system
Effects of long-term ketamine administration on rat bladder protein levels: A proteomic investigation using two-dimensional difference gel electrophoresis system
Abbreviations & Acronyms
2D-DIGE = two-dimensional difference gel electrophoresis CHAPS = 3-[(3Cholamidopropyl) dimethylammonio] propanesulfonate
Cy = cyanine dye
DIGE = difference gel electrophoresis DTT = DL-Dithiothreitol
GAPDH = glyceraldehyde
3-phosphate dehydrogenase
HK = high dose of ketamine IC = interstitial cystitis
IEF = isoelectric focusing
LK = low dose of ketamine
MALDI-TOF-MS = matrixassisted laser desorption/ionization time-of-light mass spectrometry NS = normal saline
SDS-PAGE = sodium dodecyl sulphate–polyacrylamide gel electrophoresis
Correspondence: Peng Wu
Ph.D., Department of Urology,
Nanfang Hospital, Southern
Medical University, Guangzhou
510515, China. Email: doctorwupeng@gmail.com *These authors contributed equally to this work.
Received 4 November 2012; accepted 6 January 2013.
© 2013 The Japanese Urological Association
Objectives: Long-term ketamine abuse can affect the urinary system, resulting in interstitial cystitis-like syndrome. However, its pathogenesis remains unclear. In the present study, a proteomic approach of two-dimensional difference gel electrophoresis followed by matrix-assisted laser desorption/ionization time-of-light mass spectrometry was carried out to investigate the potential disease-associated proteins in a rat model of ketamine-associated cystitis.
Methods: Rats were randomly assigned to control, normal saline, low dose of ketamine (10 mg/kg) and high-dose of ketamine (50 mg/kg) groups with six rats in each group. The two experimental groups were given ketamine hydrochloride i.p. daily, whereas the normal saline group rats were treated with saline. After 16 weeks of treatment, all bladders were excised, and samples from normal saline and high dose of ketamine groups were resolved in
Abbreviations & Acronyms
2D-DIGE = two-dimensional difference gel electrophoresis CHAPS = 3-[(3Cholamidopropyl) dimethylammonio] propanesulfonate
Cy = cyanine dye
DIGE = difference gel electrophoresis DTT = DL-Dithiothreitol
GAPDH = glyceraldehyde
3-phosphate dehydrogenase
HK = high dose of ketamine IC = interstitial cystitis
IEF = isoelectric focusing
LK = low dose of ketamine
MALDI-TOF-MS = matrixassisted laser desorption/ionization time-of-light mass spectrometry NS = normal saline
SDS-PAGE = sodium dodecyl sulphate–polyacrylamide gel electrophoresis
Correspondence: Peng Wu
Ph.D., Department of Urology,
Nanfang Hospital, Southern
Medical University, Guangzhou
510515, China. Email: doctorwupeng@gmail.com *These authors contributed equally to this work.
Received 4 November 2012; accepted 6 January 2013.
© 2013 The Japanese Urological Association
Objectives: Long-term ketamine abuse can affect the urinary system, resulting in interstitial cystitis-like syndrome. However, its pathogenesis remains unclear. In the present study, a proteomic approach of two-dimensional difference gel electrophoresis followed by matrix-assisted laser desorption/ionization time-of-light mass spectrometry was carried out to investigate the potential disease-associated proteins in a rat model of ketamine-associated cystitis.
Methods: Rats were randomly assigned to control, normal saline, low dose of ketamine (10 mg/kg) and high-dose of ketamine (50 mg/kg) groups with six rats in each group. The two experimental groups were given ketamine hydrochloride i.p. daily, whereas the normal saline group rats were treated with saline. After 16 weeks of treatment, all bladders were excised, and samples from normal saline and high dose of ketamine groups were resolved in