Pak. j. life soc. sci. (2009), 7(1):31-34
Pakistan Journal of Life and Social Sciences
Effect of Vitamin E and Selenium as Immunomodulators on Induced Aflatoxicosis in Broiler Birds Ab eer a M ub ar a k, As i f R as h id , I mt ia z Ah mad K h an a nd Az har H u ss ai n Vet eri n ar y Re sea r c h I n s tit u te, G ha zi Ro ad La ho re Ca nt t -P a ki s ta n Abstract Mycotoxicosis, a serious problem to commercial poultry, is incurable; however certain group B vitamins, vitamin E and selenium are capable of reducing and facilitating the decomposition and detoxification of mycotoxin within the body. In present study, effect of vitamin E and selenium was studied in broiler birds with induced aflatoxicosis. Five groups of broilers birds were reared and feed differently i.e., Group B was fed with vitamin E and selenium along with aflatoxin B1 mixed feed, group C was fed with vitamin E and aflatoxin B1 mixed feed, group D was fed with selenium and aflatoxin B1 mixed feed and group E was fed only aflatoxin B1 mixed feed while group A was kept as control. The study was conducted for three weeks and concluded that vitamin E and selenium in combination can act as immunomodulator even then the birds were challenged with aflatoxin B1. Keywords: Mycotoxicosis, vitamin E, selenium immunmodulator Introduction Mycotoxins are the metabolic products of toxigenic fungi, released during the growth in various feedstuffs. Aflatoxins are the most common mycotoxins, widely prevalent in different feed commodities. These toxins are considered to be most important because of wide range of host susceptibility, immunosuppression, hepatotoxicity and heat stable properties of aflatoxin and are potential hazards affecting the poultry industry resulting in heavy economic losses (Mundas and Rao, 2001). Aflatoxins are proved to suppress the immune response of the birds leading to persistence of Newcastle disease in broiler flock even after vaccination (Mani et al., 2001). Various findings strongly suggested the enhancement of immune response due to vitamin E supplementation. Vitamin E converts arachiodonic acid into prostaglandin, Correspondence Author: Abeera Mubarak, Veterinary Research Institute, Ghazi Road Lahore Cantt -Pakistan. which plays an important role in enhancement of immune response (Balker, 1993). Selenium has also been reported to improve the immune response in broilers fed with aflatoxin contaminated diets (Perozo and Rivera, 2003). Considering the importance of vitamin E and selenium in immunity, a project was designed to check the immune response of broiler birds supplemented with vitamin E and selenium alone or in combination on induced aflatoxicosis. Materials and Methods Birds Fifty, day old broiler chicks were reared and provided balance feed. The birds were vaccinated against Newcastle Disease, Hydro Pericardium Syndrome and Infectious Bursal Disease on 7th, 18th and 28th days of age, respectively. Production and estimation of Aflatoxin B1 Aflatoxin was produced by Aspergillus flavus. Briefly, 25 gm of sample was transferred to sabouraud’s medium. The sample particles were dispersed throughout the medium by shaking. The samples was incubated at 30oC and observed daily for the development of growth while the estimation of aflatoxin (part per billion) obtained from isolated culture was carried out at Department of food and Nutrition, University of Veterinary and Animal Sciences, Lahore, by thin layer liquid chromatography (Anonymous, 1990). Experimental set up The birds were divided into five groups, each comprising of ten birds i.e. A, B, C, D and E. Group A was taken as control. The birds of group B were fed with Vitamin E and Se along with aflatoxin B1 mixed feed. Group C was fed with vitamin E and aflatoxin B1 mixed feed. Group D was treated with Se and aflatoxin B1 mixed feed and group E was fed only aflatoxin B1 mixed feed. Aflatoxin B1 was given 80 parts per billion (ppb) while Vitamin E and Se (Sodium Selenite) were given...
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