Effect of Temperature on Beetroot Membrane

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 | %T at 430nm|  | |
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temperature ( °C)| Ellen| Sophie| Luke| LucyB| Will| Claire|  | Average|  | 0|  |  | 93|  |  |  |  | 93|  |
10| 82| 113| 88|  | 90| 91|  | 92.8|  |
20| 87| 90| 92| 91| 91| 89|  | 90|  |
30| 72| 85| 75| 93| 95| 89|  | 84.8|  |
40| 62| 86| 80| 82| 84| 90|  | 80.7|  |
50| 93| 87| 41| 52|  | 68|  | 68.2|  |
60| 11| 14| 10| 12| 16| 11|  | 12.3|  |
70| 11| 13| 25| 12| 10| 11|  | 13.7|  |
80| 14| 11|  |  |  |  |  | 19.5|  |
90|  |  |  |  |  | |  |  |  |
Beetroot membranes
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Results

Factor| Way of controlling/ preventing this factor influencing the outcome| Scientific reason to explain why this factor could make a difference to my results| How my results will be affected| Type of error introduced| Size/surface area of beetroot sections| I could use a size 4 cork borer to make sure all pieces of beetroot are the same size, and a ruler and knife to cut 1 cm length slices, so that they are all the same length.| It would affect my results because a larger piece of beetroot would have more pigment in it, therefore more pigment could diffuse out after proteins embedded in the cell membrane have been denatured by heat, than in other pieces. This would mean a higher absorbency of light| If my results were affected by incorrectly controlling this factor I would see inconsistencies in my results, and anomalies. Some would be higher than they are supposed to be when compared with repeats or other people’s data. So my results would be unreliable and inconcordant.| Random because it is an error in measurement of beetroot pieces that would lead to inconsistent values| Volume of water placed in colorimeter for control reading| I could use a pipette to measure out 2cm3 of distilled water into a cuvette, and then place it into the colorimeter, making sure that the light is...
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