Apparatus and materials:
1 tube mouth rinse(4 brands), 3 cylinders beetroot, 1 pc razor blade, 1 pc white tile, 10 pcs test tubes, 2 pcs test tube racks , 1pc 10-mL measuring cylinder, 5 pcs labels , 1 pc forceps, 1 pc 500-mL beaker
Aim : To study the effect of different brands of mouth rinse on the permeability of the membranes of beetroot tissue Problem: To compare the denaturation of membrane of cells in the effect of mouth rinse of different brands and find out which brand of mouth rinse damages the membrane of cells most. Therefore , the health risk of mouth rinse can be known.
denaturation is used to describe protein only, not a membrane. --> destruction
Hypothesis: The pigment in beetroot is permeable to the chemicals in the mouth rinse
The plasma membrane is made up of protein and lipid molecules. These two main components are affected by various chemicals. In this investigation, we study the degree of denaturation of the membrane of beetroot by different substances. Beetroot cells contain a water-soluble pigment, , in their vacuole which upon denaturation of membranes is released into the surrounding medium. When the beetroot discs are put into the mouth rinse and anthocyanin leaks out, the chemicals in mouth rinse are lipid-soluble molecules. The higher the concentration of the solvent, the more permeable the membrane will be. More anthocyanin from the beetroot discs will leak out.
be careful, solvent does not have a "conc."
Principle of design:
The contents of lipid-soluble substances in of mouth rinse are to be determined and compared. Four different brands of mouth rinse are bought and used for comparison. The amount of anthocyanin given out can be observed and compared after immersing into mouth rinse. Study the colour intensity find out the one contains the most lipid- soluble substances. The red pigment will leak to the surrounding solution by diffusion. Diffusion is the net movement of particles from a region of...
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