Dna Fingerprinting in Forensic Science

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“DNA Fingerprinting in Forensic Science: Isolation and Quantification of DNA and the role of RFLP and VNTR”

SUBMITTED BY
DEBOLINA GOSWAMI Roll no: BTB/10/148

SUBMITTED TO
Amity Institute of Biotechnology Amity University, Uttar Pradesh

GUIDED BY
Dr. Rachana Kumari Dr Amit Kumar Mr. K. Surendra Babu Ms. G. Jyothsna

DECLARATION

I hereby declare that the project titled “DNA Fingerprinting in Forensic Science: Isolation and Quantification of DNA and the role of RFLP and VNTR” is a record of work carried out by me, Debolina Goswami, under the supervision of Mr. Surendra Babu and Ms. G Jyothsna, Research Assisstants, Department of Molecular Biology and Biotechnology, BioAxis DNA Research Centre, L B Nagar, Hyderabad.

Place: Hyderabad Date: 22.06.2012

Debolina Goswami

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ACKNOWLEDGEMENT

William Shakespeare said “knowledge is the wing wherewith we fly to heaven”. Knowledge is rarely ever gained without the help and guidance, whether it is from inanimate objects like books, or from well-wishers and teachers. In my efforts to make this project, I, too required a lot of help and I was immensely grateful to be aided in every step of the process. I would like to express my heartfelt gratitude to Ms. Rachana Kumari, my internship guide at Amity University for giving me an opportunity to explore the intriguing world of molecular biology. I would also like to thank Dr. Amit Kumar for providing such amazing facilities for students seeking knowledge and experience. My immense gratitude goes out to Mr. Surendra Babu and Ms. G Jyothsna for helping me with every little problem that I encountered. I would also like to thank all the faculty members of Amity University who have devoted some time out of their busy schedules to evaluate this report. Last but not the least I would like to thank my friends and family for their constant support.

THANK YOU!

Debolina Goswami

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ABBREVIATION

bp DPA DPA reagent EtBr EDTA g g/l M mg ml mM N PCR RPM SDS TAE TE µl

                 

base pair Differential Producibility analysis Diphenylamine reagent Ethidium bromide Ethylene Diamine Tetra Acetic acid gram gram per liter molar milli gram milli liter milli molar normal polymerase chain reaction revolution per minute sodium dodecyl sulphate Tris Acetic EDTA Tris EDTA micro liter

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INDEX

Abstract ………..…………………………………………………………………………………7 Introduction ………….……………………………………..……………………..……..……….9 Review …………………………………………………………………………..………………17 Objective …………………………………………………….……………………..……………28 Plan of Work ………………………………….……………….……………………..………….30 Material and methods …………….…………………...................................................................32 Result and discussion …………….…………………………..……………….............................50 Conclusion ………………………................................................................................................56 Reference ……………………………………...……………………..………………..…….…..57 Appendix ……………..................................................................................................................59

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Abstract

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ABSTRACT

DNA Fingerprinting has become an indispensable tool in the world of Forensic Science. Ever since its development in 1985, this informative and highly conclusive technology has reached new heights and is now a solution to numerous problems. In this study, two basic methods of DNA Fingerprinting, i.e. Restriction Fragment Length Polymorphism and Variable Number Tandem Repeats have been covered. DNA has been isolated and purified from cheek cells, hair follicle, and blood cells. Then, this DNA has been analyzed qualitatively and quantitatively using Agarose gel electrophoresis and Differential Producibility Analysis, respectively. Then the isolated DNA has been made to undergo RFLP and VNTR. For VNTR, the DNA used was polymerized using PCR technique. This project has shown that certain methods of DNA isolation are more effective than others....
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