Diversity Analysis in Ocimum Sp. Through Rapd-Pcr.

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ABSTRACT

Tulsi is the medicinal plant widely used by traditional medical practitioners for curing various diseases in their day to day practice. In traditional systems of medicine, different parts (leaves, stem, flower, root, seeds and even whole plant) of Ocimum sanctum Linn (Tulsi), a small herb seen throughout India, have been recommended for the treatment of bronchitis, bronchial asthma, malaria, diarrhea, dysentery, skin diseases, arthritis, painful eye diseases, chronic fever, insect bite etc. The Ocimum sanctum has also been suggested to possess anti fertility, anticancer, antidiabetic, antifungal, antimicrobial, hepatoprotective, cardio protective, antiemetic, antispasmodic, analgesic, Adaptogenic and diaphoretic actions.

Medicinal properties of Tulsi (Ocimum sanctum) have been known for thousand years to various civilizations of the world. This medicinal herb is considered as a sacred plant by the Hindus in the Indian subcontinent. Scientific explorations of traditional belief of medicinal properties of Tulsi have got momentum mostly after the middle of the 20th century. In the present review, efforts have been made to sum up different aspects of scientific studies on this medicinal plant. Scientific evidences are available on various medicinal aspects Eugenol (l-hydroxy-2-methoxy-4-allylbenzene), the active constituent present in Ocimum sanctum L., has been found to be largely responsible for the therapeutic potentials of Tulsi. Although because of its great therapeutic potentials and wide occurrence in India the practitioners of traditional systems of medicine have been using Ocimum sanctum L. for curing various ailments, a rational approach to this traditional medical practice with modern system of medicine is, however, not much available. In order to establish the therapeutic uses of Ocimum sanctum L. in modern medicine, in last few decades several Indian scientists and researchers have studied the pharmacological effects of steam distilled, petroleum ether & benzene extracts of various parts of Tulsi plant and eugenol on immune system, reproductive system, central nervous system, cardiovascular system, gastric system, urinary system and blood biochemistry and have described the therapeutic significance of Tulsi in management of various ailments. These pharmacological studies have established a scientific basis for therapeutic uses of this plant.

CONTENTS
S. NO.| PARTICULARS| PAGE NO.|
1.| INTRODUCTION| |
2.| REVIEW OF LITERATURE| |
3.| MATERIALS AND METHODS| |
4.| RESULTS | |
5.| DISCUSSION| |
6.| SUMMARY AND CONCLUSION| |
*| BIBLIOGRAPHY| |
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ABBREVIATIONS

A260| -| Absorbance at 260 nm|
oC| -| Degree centigrade|
µg| -| Microgram|
µl| -| Microliter|
µM| -| Micro molar|
A280| -| Absorbance at 280 nm|
BME| -| -mercaptoethanol|
bp| -| Base pairs|
CTAB| -| Cetyl Tremethyl Ammonium Bromide|
d.d.W| -| Distilled Deionised Water|
DNA| -| Deoxyribonucleic Acid|
dNTPs| -| Deoxynucleotide triphosphate|
EDTA| -| Disodium ethylenediamine tetra acetate|
g| -| Grams|
g/l| -| Gram per litre|
Kb| -| Kilobase pairs|
Kd| -| Kilo Dalton|
Liq.N2| -| Liquid Nitrogen|
M| -| Molar|
mg| -| Milligram|
mg/l| -| Milligram per Liter|
ml| -| Millilitres|
mM| -| Millimolar|
Mol.wt.| -| Molecular Weight|
NaCl| -| Sodium Chloride|
Ng| -| Nanograms|
nm| -| Nanometers|
OD| -| Optical Density|
PCR| -| Polymerase Chain Reaction|
Pp| -| Polypropylene Tubes|
RNA| -| Ribonucleic Acid|
RNase| -| Ribonuclease Enzymes|
rpm| -| Rotation per minute|
TBE| -| Tris Borate EDTA|
UV| -| Ultra Violet|
V| -| Volts|
| -| Lambda|

INTRODUCTION

Tulsi is considered to be a ubiquitous plant in India. The name Tulsi is derived from ‘Sanskrit’, which means “matchless one”. Tulsi plays a...
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