Difco Manual

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Difco & BBL Manual
Manual of Microbiological Culture Media Second Edition

Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152

Copyright 2009 Becton, Dickinson and Company 7 Loveton Circle P.O. Box 999 Sparks, Maryland 21152

ISBN 0-9727207-1-5 All rights reserved Printed in the United States of America

AOAC is a trademark and Performance Tested Methods is a service mark of AOAC International. ATCC is a trademark of the American Type Culture Collection. CHROMagar is a trademark of Dr. A. Rambach. Bacto, BiTek and Difco are trademarks of Difco Laboratories, Inc., subsidiary of Becton, Dickinson and Company. Unless otherwise noted, BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. 2009 BD.

Table of Contents

Contents
Preface ...............................................................................................................................................................v About This Manual ...........................................................................................................................................vii History of BD Diagnostics .................................................................................................................................ix Section I: Monographs .......................................................................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media ..................................................................................................................5 Culture Media Ingredients – Agars ...................................................................................................................6 Culture Media Ingredients – Peptones and Hydrolysates..................................................................................7 Media Sterilization .........................................................................................................................................13 Quality Control Organisms............................................................................................................................15 Typical Analyses.............................................................................................................................................17 Section II: General Technical Information ........................................................................................................19 Dehydrated Culture Media ............................................................................................................................21 Prepared Plated Media ...................................................................................................................................25 Prepared Tubed, Bottled and Mycoflask™ Media ............................................................................................27 Section III: Culture Media and Ingredients .......................................................................................................31 Section IV: Reference Guide ...........................................................................................................................635 Culture Media for Specific Groups of Microorganisms ................................................................................637 Application Tables...
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