Diagnosis of Diseases

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SUBMITTED BY:-
PRACHI SINGH
B.TECH BIOTECHNOLOGY
SEMESTER VIII
A7104108153

Project on
Basic Techniques
Submitted to: Submitted by: Mrs. Preeti Mathur Prachi Singh A7104108153 B.tech (Biotech) 5th Sem Sec - ‘C’ Roll no. - ‘27 A DISSERTATION ON

A Project On The Lab. Diagnosis of SEXUALLY TRANSMITTED DISEASES (HIV, HBsAg, HCV) Submitted in partial fulfillment for the award of 4 years program B.Tech Biotechnology degree

To
Amity institute of Biotechnology (AIB)

Project Undertaken at 
SUPER RELIGARE LABS (FORTIS Hospital,NOIDA)
                                     Under the Supervision of : Dr. Rajeev Tangri
                      MD. Super Religare Laboratories (Fortis, Noida)   
 
 Under supervision of                                                 Compiled by Dr. Rajeev Tangri                                                   Prachi Singh B.Tech Biotechnology  ACKNOWLEDGEMENT

        It gives me immense pleasure to express my gratitude to all those who have accompanied/supported me during this research work.        I express my deepest gratitude to Mr. C.Kumar without whose kind co-operation this project would not have been possible.        I am indebted to reverend Dr. Jaya, my esteemed supervisor at SRL Laboratories, for giving me an opportunity to work under her guidance. Her constant support throughout the training made it a memorable experience at the Hospital.        I take this opportunity to express my heartfelt gratitude to Dr. Rajeev Tangri for his support and cooperation to carry out the research work.         

 
Date:21/05/2012                                                    Prachi Singh

DECLARATION

I Prachi Singh hereby declare that One Month Training Programme In Molecular Biology is a record of original work done by me under the guidance of Dr. Riyazul Hasan Khan, Director, Genetech Laboratories and under the supervision of Mr. Nasiruddin Alam. I also declare that no part of this thesis has previously been submitted to any University or any examining body for acquiring any diploma or degree.

DATE: 21/05/2012 NAME: Prachi Singh

CONTENTS

S.NO.| TOPIC|
1.| ABBREVIATIONS|
2. | LABORATORY RULES & REGULATIONS|
3. | APPARATUS USED IN LABORATORY|
4.| PREPARATION OF PURE BACTERIAL CULTURE|
5.| GENOMIC DNA ISOLATION FROM BACTERIAL CULTURE|
6.| GENOMIC DNA ISOLATION FROM HUMAN BLOOD CELLS|
7.| PLASMID DNA ISOLATION FROM BACTERIAL CULTURE|
8.| DNA ISOLATION ( CTAB METHOD )|
9.| AGAROSE GEL ELECTROPHORESIS|
10.| SDS-PAGE|
11.| RESTRICTION|
12.| LIGATION|
13.| ELUTION|
14.| QUANTIFICATION|
15.| DNA AMPLIFICATION BY PCR METHOD|

ABSTRACT:

INTRODUCTION:
Serology is the scientific study of blood serum and other bodily fluids. In practice, the...
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