DEVELOPMENT OF CONTINUOUS CELL LINE
ANN ANU KURIAN
I take this opportunity to present my thanks to all those guidepost who really acted as lightening pillars to enlighten their way throughout this project that has led to successful and satisfactory completion of this study.
I am highly thankful to Dr. SHEELENDRA M. BHATT for his active support, valuable time and advice, whole-hearted guidance, sincere cooperation and pains taking involvement during the study and in completing the assignment of preparing the said project within the time stipulated.
Lastly, I am thankful to all those, particularly the various friends , who have been instrumental in creating proper, healthy and conductive environment and including new and fresh innovative ideas for us during the project, their help, it would have been extremely difficult for me to prepare the project in a time bound framework.
ANN ANU KURIAN
3)MORPHOLOGY OF CELLS IN CULTURE
4)TYPE OF CELL CULTURE
6) FINITE V/S CONTIONOUS
7)ADHERENT CELL CULTURE V/S SUSPENSION CELL CULTURE
8)CRITERIA FOR CELL LINE SELECTION
9)CHARACTERSTICS OF CONTINOUS CELL LINE
10)PROPERTIES OF CONTINOUS CELL LINES
11)EFFECT OF CELLULAR ENVIRONMENT ON
CONTIONUS CELL LINE
12)DEVELOPMENT OF CONTINIOUS CELL LINE
13)BASIC COMPONENTS IN CULTURE MEDIA
14)PATHWAY LEADING TO CONTINOUS CELL
15)MAINTANINACE OF CONTIONOUS CELL LINES
16)TEST FOR CHECKING CONTIONOUS CELL LINE
17)COMMERICALLY IMPORTANT CELL LINES
18)APPLICATION OF CONTIOOUS CELL LINES
19) DISADVANTAGES OF CONTINOUS CELL LINES
Cell culture is the technique to maintain and grow cells in vitro outside of their original environments. Cell culture has been used extensively as a model system for biology, disease, and therapeutics. It is applied across the disciplines of genetics, immunology, cancer, medicine, vaccine production, tissue engineering, and countless other applications.
The cell culture technique was developed over a century ago by Dr. Ross Granville Harrison in 1907 . Harrison was working in experimental embryology and devised a method to culture and observe frog embryo nerve fiber growth in vitro. This influential technique was gradually refined, and used in primary explants for the next 50 years. Several developments in the 1950s popularized the cell culture technique. The use of trypsin allowed adherent cells to be dissociated from surface so subculturing of cell lines can be done conveniently. The use of cells to grow virus and use the virus for vaccine production has driven advancement in cell culture technology. Development of antibiotics made it easier to prevent the cell culture from contamination.
One major breakthrough occurred in 1952 with the establishment of the first human cell line, HeLa. George Otto Gey used cervical cancer tissues from patient, Henrietta Lacks (hence the name HeLa), to create an immortal cell line. This laid the foundation for research in this field as HeLa rapidly became adopted in numerous labs for cancer studies and polio vaccine testing and development. The ability to grow human cells also stimulated interest in the use of cells as biological models...