Determining the Factors that Positively Influence Catalase Reaction Rates in the Breakdown of Hydrogen Peroxide

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This experiment was performed to determine the factors that positively influence catalase reaction rates in the break down of hydrogen peroxide. Catalase enzyme activity was measured through its break down of hydrogen peroxide into water and oxygen through the displacement of water. We compared the decomposition rates in samples with varying pH levels of 2-10. The samples with the most neutral ph level decomposed hydrogen peroxide the quickest and most efficiently.

Catalase is a fast working enzyme that breaks down hydrogen peroxide into water and gas.( Many factors affect the break down of hydrogen peroxide, one of the main factors that relates to the human body is pH levels. With the presence of strong acids or strong bases the production rate of oxygen gas will increase the rate of reaction. As quoted from “The optimal pH range is about 7-8 (physiological pH of most cells), but a few enzymes can work at extreme pH, such as protease enzymes in animal stomachs, which have an optimum of pH 1.” This shows that for the human body catalase needs to be able to function properly at extreme pH levels, proving where our hypothesis was drawn from.

Record the pH level of the mix between catalase and the acid/base Dip the circles of filter paper into the mixture and place on top of the inside of the glass bottle. Add 5mL of hydrogen peroxide into the glass bottle ensuring that none touches the opening or the filter paper. Place the bottle in the water tray and slide the nossel under the graduated cylinder full of water. Flip the bottle so the hydrogen peroxide mixes with the catalase filter paper. Measure the production of oxygen gas every 5 seconds for 1 minute. Repeat for the remaining pH levels.

-Sulphuric Acid (0.05M)
-Potassium hydroxide (0.05M and 0.5M)
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