Comparing Fermentation Rate of the S. Cerevisiae Yeast in the Presence of Mgso4, Naf and Sodium Pyruvate

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Syl Rogers
Bio 210

Comparing Fermentation Rate of the S. cerevisiae Yeast in the presence of MgSO4, NaF and Sodium Pyruvate

Hypothesis
In the fermentation of rate of yeast, S. Cerevisiae, there will be a higher/ faster rate of ethanol production, However, using catalytic enzymes would make the rate more faster, and MgSo4 will have a higher rate of CO2 than that of NaF and Sodium pyruvate as it act as a more better catalytic enzyme than the others.

Methods
Preparation of Tubes
A solution of yeast and glucose was prepared with different concentration of enzymes. In All the tubes water, 0.3M glucose and yeast was placed; with the exception of some tubes in which 0.2M MgSo4 and 2.5mL and 1.4ML 0.2 NaF was placed respectively. In another tube was placed both MgSO4 and NaF. The different solutions were prepared and placed in separate tubes for the experiment. A 45oC water bath was setup to be used for the fermentation process. Data acquiring

The tubes were placed in the water bath. A stop watch was used which aids in determining the reaction time. After Every 15 min, data was collected from all tubes by measuring the amount of CO2 being produced. This process continued for a total of 75min allowing enough time for Fermentation to occur in all tubes.

Result
For the first 15min, In the tube containing 8.5mL water and 2.5mL yeast only, the rate of the reaction was 0mm/min whiles the tube with the glucose has a rate of 233.864mm/min. This is followed by the tube containing 2.5mL of MgSo4 which has a rate of 165.8573mm/min. The tube containing the MgSo4 and NaF has a rate of 69.85mm/min which is followed by the 1.24mL NaF tube that has a rate of 40.63mm/min followed by 2.5mL NaF tube which has a rate of 31.08mm/min which was the lowest. After 30minutes, the tube containing only water and yeast has a rate of 0mm/min whiles the tube with the glucose has a rate of 208.97mm/min. This is followed by the tube containing the MgSo4 which has a rate of...
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