Comparing Dna Sequences to Understand Evolutionary Relationships with Blast

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Name: Rimsha Ahmed
John F Kennedy HS
Dr. Fisher
Date: 1-14-13
Block: 3

I. Comparing DNA Sequences to Understand Evolutionary Relationships with BLAST:

Mental disorders are not present in animals.
As much as life has evolved, the relationship between animals and humans has remained very close to each other. If animals are, evolutionally, and genetically similar to humans; can they develop the same diseases we have today? Humans today have mental disorders left and right: OCD(obsessive compulsive disorder), autism, ADD (attention deficient disorder), bipolar disorder, depression, insomnia, anxiety. If all these diseases occur in the minds of humans, can they occur in the minds of animals also? Dogs share approximately 75% of their DNA with humans; can it occur in them too? A lacking in the protein called SAPAP3 is believed to be the cause of OCD-related symptoms in mice. If the protein SAPAP3 is found in other organisms, can they be susceptible to mental disorders similar to OCD? If the protein named SAPAP3 is found in dogs, coyotes, wolves, foxes (taxid:9608) at more than 75% identical, then these animals are vulnerable to mental disorders similar to OCD.



These are our top 5 results. The first one is nearly identical to the gene, while the bottom four are barely half similar.

The most similar protein was found in Canis lupis familiaris. It is 96% identical and 97% similar. The E value is 0.0.

The second most familiar protein was also found in Canis lupis familiaris. This genome is significantly less similar. It is 47% identical, 57% similar, and has an E Value of 0.0.

The first result has an almost entirely identical genome as the one found to cause OCD- like symptoms in rats. This signifies, theoretically, that if there were to be an alteration or lacking in the genome in Canis lupis familiaris, the OCD-like symptoms found in mice could also be found in this organism. The second result was very different from the first result. Even though it had an E value of 0.0, it is only 47% identical and 57% similar. This is almost half the first result. 47% and 57% is a lot, but it is too unsimilar to code for a functioning protein that can cause a mental disorder when corrupted. The mice tested had a lacking of SAPAP3 in their DNA. This can also mean that this organism, Canis lupis familiarlis, has inborn obsessive compulsive habits. This is a proposed cladogram in relationship to Homo sapians, Mus musculus, and Canis lupis familiarlis in accordance to the SAPAP3 gene.

 Lab Questions:
1. Q: What species in the BLAST result has the most similar gene sequence to the gene of interest? A) Gallus gallus collagen, type V, alpha 1 (COL5A1), mRNA.
2. Q: Where is that species located on your cladogram?
A) It is located on a same branch and has the closest common ancestor compared to all the other organisms of the cladogram. 3. Q: How similar is that gene sequence?
A) That gene sequence is 100% similar.
4. Q: What species has the next most similar gene sequence to the gene of interest? A) Meleagris gallopavo collagen alpha-1(V) chain-like (LOC100545544), miscRNA. 5. Q: Compare and discuss your cladogram with your classmates. Does everyone agree with the placement of the fossil specimen? If not, what is the basis of the disagreement? A) Everyone agreed for the most part. There were some disagreements about the location of the fossil on the cladogram but that was because we did not all have the same knowlage anout fossils and the other organisms. 6. Q: On the main page of BLAST, click on the link “List All Genomic Databases”. How many genomes are currently available for making comparisons using BLAST? How does this limitation impact the proper analysis of the gene data used in this lab? A) There are 254 genomes currently available for making comparisons using BLAST. 7. Q: What other data...
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