Comparative Study on Regeneration Potentiality of Aromatic Indica Rice (Oryza sativa L.) of Bangaldesh Golam Gaus Mohiuddin Chowdhury1, M. Ashrafuzzaman*1, F. Raihan2 and Shamsul H. Prodhan1 1
Department of Genetic Engineering and Biotechmology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh 2 Department of Forestry and Environmental Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh *E-Mail: email@example.com ; firstname.lastname@example.org
In this study, two varieties of aromatic rice (Oryza sativa L.) BRRI Dhan 50 (Bangla Moti) and BRRI Dhan 34 (Khaskhani) were used to establish a suitable system for callus initiation and regeneration. MS (Murashige & Skoog, 1962) media supplemented with different concentrations i.e. (1.0, 2.0, 3.0, 4.0 mg/L of 2,4-D (2,4-dichlorophenoxyacetic acid) and combination with BAP (6-benzylaminopurine), (2.0 mg/L) were used for callus induction from mature dehusked rice seeds. MS medium supplemented with only 3.0 mg/L of 2,4-D, produced maximum percentage of callus that is 90% for BRRI Dhan 50 and 80% for BRRI Dhan 34. On the other hand, MS media with 3.0 and 4.0 mg/L of 2,4-D in combination with 2.0 mg/L BAP produced highest percentage of callus (80 %) for BRRI Dhan 50 and 70 % for BRRI Dhan 34. For plantlet regeneration, MS media with 1.0 mg/L NAA (1-Napthaleneacetic acid), 2.0 mg/L BA (6-benzyladenine) and various concentrations of Kinetin (0.0, 1.0, 2.0, 3.0, 4.0 mg/L) were employed. The maximum percentage of shoot regeneration was recorded at MS media supplemented with 4.0 mg/L of Kinetin +1.0 mg/L NAA and 2.0 mg/L BA for both varieties. These results will be very helpful to improve rice quality through somaclonal variation and genetic transformation. Keywords: Auxin, BRRI Dhan, Cytokinin, Plantlet Regeneration, Aromatic Rice, Oryza sativa
Rice, like wheat, corn, rye, oats and barley belongs to Gramineae or grass family. Fine rice, is a part of the rice family (Oryza sativa L.). In Bangladesh, there are about more than 7,000 varieties of rice are grown in various parts of the country. Nearly 70% of the land area of the country has been brought under rice cultivation. Out of this 70%, fine rice is cultivated on roughly 10% land. This lower coverage is primarily due to the emphasis of government policy and research on food grain production but with low input technology. Fine rice production reached 28 million tons in 1993 but has declined marginally over the last three years due to consecutive draughts and floods. It is observed that the fine rice is a profitable farming venture for the farmers, as its cultivation does not normally require additional expenditure on fertilizer, pesticides and irrigation, and a good source of livelihood. Rather using indiscriminate use of fertilizers increases the thickness of the rice and reduces aroma. The use of organic fertilizers and pesticides, through traditional practices, believes to help enhancement of aroma and preservation of fineness as mentioned by the farmers. The average yield of fine rice is about 2.6 tons/hectare and has increased at a rate of 2.65 tons/hectare per year from 1980 to 1992 (Islam et al, 1996). Plant cells possess totipotency, i.e., whole plants can be regenerated from single cells by modulating culture conditions (Steward et al, 1958). The mechanisms of totipotency, however, are little understood so far, and are mainly discussed in relation to the concentration and ratio
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M. Ashrafuzzaman et al / Research Journal of Biology (2012), Vol. 02, Issue 03, pp. 98-103
of phytohormones (Toonen & De Vries, 1996). Plant tissue culture is a practice used to propagate plants under sterile conditions, often to produce...