5.05 LAB REPORT
Paper chromatography is a widely used method of separation. The lab will show the basic techniques of paper chromatography. In this lab, the separation of the dyes used in two different kinds of candy is performed. The candy dyes may also be compared to solutions of FD&C food dyes.
The purpose of this experiment is to separate and compare dyes found in two different kinds of candy.
• Candy with a colored coating, like Skittles® or M&Ms® (4 different colors) • Rubbing alcohol or isopropyl alcohol
• Coffee filters (2)
• Tall glasses or plastic cups (2)
• Foil or paper plate
• Table salt
• Toothpicks or cotton swabs (4)
• Measuring cups/spoons
• Clean pitcher or 2-liter bottle
1. Coffee filters usually are round, but it's easier to compare your results if the paper is rectangular. So your first task is to cut the coffee filter into a rectangle measuring three cm by nine cm. You will need two of these strips to complete the lab.
2. Space four drops of water (or however many colors you are testing) equally distant on a piece of foil or plate. Position one candy of each color on the drops. Give the color about a minute to come off into the water. Dispose of the candy.
3. Using a pencil (do not use a pen), draw a line one cm (1/2 inch) from the edge of one end of one strip of paper.
4. Make four pencil dots (one for each color of candy you will be examining) along this line, about 0.5 cm (1/4 inch) apart. Underneath each dot, label the color of the candy you will test on that spot. You won't have space to write the whole color name, so try abbreviations like B for blue, G for green, etc. 5. Dip a toothpick or cotton...