Cmmb Notes

05-09-13

CMMB 511

No final exam
Journal based course.

Try to think of everything in terms of GENE EXPRESSION

Biol questions

1) concepts
2) strategies and approaches – need cross f(x) or complimentary approaches to develop a compreshensive understanding
3) techniques – lots of techniques, make sure using appropriate techniques
4) outcome of results
5) overall conclusions

DNA content per haploid genome:

Bacteria: 2 x 106 bp
Mammal: 2 x 109 bp

Complexity of the genomes. Important question in biol scinces is

1) How is the genome organized?
2) How is gene expression regulated? Very tightly regulated, don’t expect genes to go up or doen. Different mechanisms, remarkable complexity.

These are important questions in the understanding of cellular processes including:
cell differentiation, development, diseases (etc). the study of processes comes down to the two above questions. If you know the basic concepts, can answer anything!

05-09-15

How is the genome organized?

How do we know the presence of the sequences. Will re-visit classic methods to determine the sequence of these genomes.

S(X) of the chromo
Each chromo is a single, linear DNA molecule

At the ends are the telomeres – f(x) in the repication, make the chromosome tips inert to chemical interactions and the enqumatic attach

A chromo is divided in to 2 arms by the centromere – the position at which spindle microtubule attach to the chromosome during cell division

Chromo have sites serving as replication origins

Chromatin – (DNA and PROTEIN ) organization of the chromosomes

Genome activity – linear CHROMATIN that is tc’d not the DNA

Morphological complexity – determined by the DNA content in the haploid genome (AKA genomic complexity) , simplest is the micoplasma very complex are flowering plants. The nubmer of bp present in E.coli I sfar less than that in flowering plants, therefore when we talk about genomic complexity – it’s the number of bp... [continues]

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