Chromatography Lab Repot

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Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT

CHEMISTRY LAB REPORT

Name: Sri Danielle
Class: 9A
Teacher: Sir Ajay
Name: Sri Danielle
Class: 9A
Teacher: Sir Ajay

Aim
My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography.

Hypothesis
I think that there will be a high RF value in paper chromatography, if we use water solvent because a solvent that has a strong interaction with a chemical will easily over come attraction from the chemical for the absorbent layer and move that chemical in a given period of time.

Materials
Food Coloring (solute)
Ethanol, Acetone, Tap Water, Distilled Water(solvents)
Pencil
Ruler
Scissors
Filter Paper
Petri Dish

Variables
Independent: Type of chromatography solvent
Dependent: Retention in paper chromatography
Controlled: Type of Chromatography paper
Size of spot
Thickness of Chromatography
Substance tested for paper chromatography
Concentration of the solute
Temperature

Variables Manipulation

Independent: Apply different solvents
Dependent: The RF value of the given solvent
Controlled: Keep the solute same.
Keep the size of the solution the same (one drop)
Keep the temperature of the room the same.

Diagram

Method

Step 1 – Prepare Materials and pour solvents into the petri dish. Step 2- Fold the provided chromatography paper into half (make sure it can stand) Step 3- measure 1.5cm from one end of the paper then draw a line across. This will be used as a starting point. Step 4- Repeat for 4 chromatography papers for each solvent. Step 5- Place a drop of the solution on the pencil line for all 4 chromatography...
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