Preview

Chromatography Experiment of the Drosophila Eye

Better Essays
Open Document
Open Document
1226 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Chromatography Experiment of the Drosophila Eye
Chromatography Experiment of the Drosophila Eye Pigmentation Pathway
Introduction:
How can the use of chromatography help explain single gene mutations of the drosophila melanogaster bio-synthetic pigment pathway? This experiment was meant to help connect the mode of inheritance with different eye color mutations and pinpoint where they occur in the pigment pathway. The drosophila eye color is a result of two bio-synthetic eye color pathways, this is expressed as a trait or phenotype, a multigene trait. There is the ommochrome pathway that produces a brown pigment and the drosopterin pathway that results in the bright red pig mention due to many pigments blended together. The initial substrates or precursors begin with the amino acid tryptophan into ommochrome and guanine into the pterin pathway is an enzymatic pathway is allowed to continue creating one enzymatic reaction after another, resulting in the end color produced. Together if these two pathways have no mutations, this results in the wildtype eye color that is brick red. Wildtype color is the dominant eye color for the fruit fly or what is normally seen in the population. If the eye color is other than wildtype this indicates that there is a mutation in either pathway that produces pigmentation’s. This means there is a defect in the gene that encodes for an enzyme in either pathway or it may mean that there is a mutated gene that codes for the pigment transport system that affects the overall eye color. (Bio 2.1 lab manual pg. 133.)

The molecular biology class of Santa Rosa Junior college have been undergoing experiments using chromatography to further study eye pigment mutations of the drosophilia, knowing that there are two different pathways linked to the different colors. Five different colors were studied; wildtype, scarlet, brown, sepia, and white. Hypothesis of how these colors are linked to the enzymatic pigment pathways went as follows: the wildtype had both functional bio-synthetic



References: 1)“Drosophila Chromatography.” Bio-2.1 Laboratory Manual. Santa Rosa Junior College Fall 2010.(pg.147-151) 2)“Drosophila Lab Part 1.” Bio-2.1 Laboratory Manual. Santa Rosa Junior College Fall 2010.(pg 132-134) 3)Hodge, S. (1981) Some epistatic two-locus models of disease. I. Relative risks and identity by descent distributions in affected sib pairs. Am. J. Hum. Genet.,33,382–395

You May Also Find These Documents Helpful

  • Powerful Essays

    The purpose of this experiment was to explain how colorimetry can be used to qualitatively detect cellular chemical components; to chemically differentiate between proteins, sugars, starches, and lipids; to identify the roles of molecular components in living systems; to comprehend the value of using a systematic approach to research; and to describe why hypotheses, controls, standards, and quality control are important in scientific research (Vorndam, 2002, p. 92).…

    • 7879 Words
    • 32 Pages
    Powerful Essays
  • Powerful Essays

    This experiment focuses specifically on Drosophila melanogaster, also known as the common fruit fly (Lynch, M., et al. pp 645-663). For over 100 years scientists have been using them in experiments as they are referred to as a “model organism.” They were deemed a “model organism” since they are characterized as…

    • 2415 Words
    • 10 Pages
    Powerful Essays
  • Better Essays

    Genetics Cross

    • 2548 Words
    • 11 Pages

    Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans…

    • 2548 Words
    • 11 Pages
    Better Essays
  • Better Essays

    Allele and Probability

    • 1288 Words
    • 5 Pages

    (a) List the possible genotypes of their offspring. (a) Sample space is {SS,Ss,sS,ss} where S=dominant disease allele and s=normal recessive allele…

    • 1288 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Family Genetic Guidelines

    • 920 Words
    • 4 Pages

    This assignment is to help you gain insight regarding the influence of genetics on an individual’s health and risk for disease. You are to obtain a family genetic history on a willing, non-related, adult participant.…

    • 920 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    High School Fruit Fly Lab

    • 3600 Words
    • 15 Pages

    The idea of this experiment is to give a clearer understanding between autosomal, sex-linked, recessive and dominant genes. Furthermore, with this knowledge we can then understand how a gene is passed down through its generations and why it would appear in one individual and not in another. The experiment also helps give a better understanding of genotypes and phenotypes and what role they play in determining and predicting a genotype.…

    • 3600 Words
    • 15 Pages
    Powerful Essays
  • Better Essays

    Fruit Fly Genetics

    • 1534 Words
    • 7 Pages

    McKusick, Victor A. Mendelian Inheritance in Man: Catalogs of Autosomal Dominant, Autosomal Recessive, and X-linked Phenotypes. Baltimore: Johns Hopkins UP, 1983. Print.…

    • 1534 Words
    • 7 Pages
    Better Essays
  • Better Essays

    life cycle of four specific stages. The first stage is the egg, which is about .…

    • 1698 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Fruit Fly Lab

    • 617 Words
    • 3 Pages

    The major topic of this experiment was to examine two different crosses between Drosophila fruit flies and to determine how many flies of each phenotype were produced. Phenotype refers to an individual's appearance, where as genotype refers to an individual's genes. The basic law of genetics that was examined in this lab was formulated by a man often times called the "father of genetics," Gregor Mendel. He determined that individuals have two alternate forms of a gene, referred to as two alleles. An individual can me homozygous dominant (two dominant alleles, AA), homozygous recessive, (two recessive alleles, aa), or heterozygous (one dominant and one recessive allele, Aa). There were tow particular crosses that took place in this experiment. The first cross-performed was Ebony Bodies versus Vestigle Wings, where Long wings are dominant over short wings and normal bodies are dominant over black bodies. The other cross that was performed was White versus Wild where red eyes in fruit flies are dominant over white eyes.…

    • 617 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Sordaria Lab Report

    • 1466 Words
    • 6 Pages

    -Is there any variation in the ratio of the two crossover types between gray and tan spore color genes? There was not a significant difference in the ratios of the crossover types between the gray and tan spore color genes.…

    • 1466 Words
    • 6 Pages
    Powerful Essays
  • Powerful Essays

    Robinson, T. R. (2005). Genetics for Dummies (2nd ed. pp. 142). Hoboken: Wiley Publishing, Inc.…

    • 2156 Words
    • 9 Pages
    Powerful Essays
  • Powerful Essays

    afull description of both HC and SC with a labeled diagram that includes gene/allele sequences for a heterozygote and ‘m’ or ‘p’ labels to indicate parental origin;…

    • 1862 Words
    • 6 Pages
    Powerful Essays
  • Better Essays

    Our TLC plate showed all colours expressed by the pteridine pigments for the wild-type Drosophila as expected (Figure 1). The sample A mutant which was a bright-red eye mutant also expressed the same pigments as our wild-type. Compared to the wild type, sample A pigment spots were lower intensity in colour under the UV light (Figure 2). Sample B was a brown-eye phenotype that showed no pigments except for a very faint 2-amino-4-hydroxypterin pigment (Figure 2). This was probably the result of human error because it was expected that there would be no pigments shown on the TLC plate for the brown-eye mutant. Furthermore, sample C which was the white-eyed mutant where no pigments were expected; however, our actual results did not reflect this. Instead our TLC plate faintly expressed Isoxanthopterin (violet-blue) for the white eye mutant (Figure 2). This was most likely due to human error. Moreover, the pigments found for sample D which was the dark-brown grey phenotype were: Isosepiapterin, Biopterin, 2-amino-4-hydroxypterin, and Isoxanthopterin and was lacking Sepiapterin, Xanthopterin, and Drosopterin (Figure 1). The Sepiapterin and Xanthopterin pigment was expected to show on the TLC plate (Figure 1) but unfortunately our plate did not reflect this. Again, this could be due to human error during the experiment. The Rf values for the pteridine pigment spots were similar for both the wild type and mutants of Drosophila. The wild type and sample A (bright-red eye mutant) had very similar Rf values for all the pigments (Table 1). Conversely, the Rf values for Isosepiapterin, Isoxanthopterin and Sepiapterin for the wild-type were slightly higher than those same pigments of sample A (Table 1). The 2-amino-4-hydroxypterin spot for sample B (Brown-eye) was close in value to the other…

    • 1483 Words
    • 6 Pages
    Better Essays
  • Good Essays

    In this article, we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs.…

    • 645 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    To control for color change of the mitochondrial enzymes alone without addition of NADI. (false +)…

    • 592 Words
    • 3 Pages
    Satisfactory Essays