1.analytical: used to measure ratios of analytes(substance in simpler forms) in a mixture. Example- analyte 1: analyte 2 = 1:2 2.preparative: for studying on a higher level
There are three main components of which a chromatographic experiment consists of, these are the: 1.Mobile Phase: solvent into which sample is dissolved and helps sample travel through stationary phase. It is mobile. 2.Stationary Phase: Permanent part of chromatographic procedure, through which the mobile phase moves. It is stationary i.e. it doesn’t move. 3.Sample: This is the solution that has to be examined
Chromatography is a universal tool, which we can use to separate even the most similar substances, volatile or soluble, but only if you have the correct: 1.absorbing materials
Year NameContribution Progress
1900Mikhail Tsvet (Russian Scientist)Idea is first conceivedMainly separated plant pigments (chlorophyll, carotenes and xanthophylls). these each were coloured differently, thus retaining the name chromatography 1930-
1940Technique became more and more globalised
1940Archer John Porter and Richard Laurence Millington SyngeDeveloped rules and methods to be followed out during processTheir work lead to the establishment of: gas, paper and high performance liquid chromatography
1978W C StillIntroduces Flash Column Chromatography (faster more efficient version of column chromatography)Separation of mixtures into analytes can be performed within 20 minutes and is sold on mass...