Chemical Aspects of life
in this section i will be discussing my thoughts of the chemical aspects of life. Explaining what my hypotheses are, for what chemicals are in which substances and what affects the reagents will have on them. Protein testing will be performed on 2 substances,egg albumin and gelatin using Biuret's solution. If biuret's solution is added to egg albumin then the egg albumin will change colors. If biuret's solution is added to gelatin then there will be only the color of the biuret's solution in there. When testing for lipids using the grease spot test three substances will be left to dry on a brown paper bag square lipids will appear in the form of a stain on the paper bag. If oil is left to dry on a paper bag then very evident stain will appear. If milk is left to dry on a paper bag then a residue will be left on top of the bag. When testing for lipids Using Sudan IV an oil and water test will be conducted, and an milk and water test will be done. If oil and water are tested using sudan IV then the oil will mix with the sudan IV. If milk and water are tested using Sudan IV then the Sudan Iv will not mix with either. When testing for carbohydrates benedict's solution will be used and Hcl will be used as an additive to alter results. If benedict's is added to glucose then the solution will change color. If benedict's solution is added to sucrose then the solution will change color. If benedict's solution is added to sucrose and Hcl there will be a more drastic color change. If benedict's solution is added to milk then there will be a slight color change. If benedict's solution is added to Hcl and milk there will be a drastic color change. If benedict's solution is added to starch then there will be a color change. If benedict's solution is added to starch and Hcl then there will be a more drastic color change. When testing for carbohydrates using iodine a porcelain spot plate will be used to better see color changes. If iodine is added to a starch solution then it will change color. If iodine is added to water then the iodine will become dilute. PROCEDURE
Procedure: You will be testing for the presence of the following subtances: proteins, lipids, and carbohydrates. The carbohydrates will include monocaccharides (glucose), disaccharides (sucrose), and polysaccharides (starch). Protein test
Background: Proteins give color reactions with certain reagents. The compounds that give rise to these colors are formed not by the whole protein molecule but by certain amino acids present in the protein. Biuret solution will be used for the test. Biuret solution is a blue solution that turns a violet color in the presence of proteins this color change occurs when the Biurets reacts with the amino groups found in the amino acids that are the building blocks of proteins. add 3ml of dilute egg albumin solution to a test tube. Add biuret's solutiong drop by drop. Stop if a violet color is obtained. Do not continue until a blue color occurs repeat the test with gelatin. Record your results.
Background: Lipids are insoluble in water but are soluble in fat solvents such as ether, acetone, and carbon tetrachloride. The simplest lipids are composed of carbon, hydrogen and oxygen. Lipids will remain on a brown paper bag after the water in the solution has evaporated, this will make the bag somewhat transparent. Secondly, a dye test will be done. In this test, dark red sudan IV will be used. Sudan IV is not soluble in water, but is soluble in lipids. You will be observing the distribution of dye in this test. Procedure:
with a medicine dropper, add a drop of salad oil to the corner of a brown paper bag. To the opposite corner, add a drop of water. To one more corner, add a drop of milk. Let the fluids evaporate and then examine each spot by...
Please join StudyMode to read the full document