Chapter 12, Question.

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  • Topic: ELISA, Antibody, Western blot
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REVIEW SHEET
EXERCISE 12
Serological Testing

NAME: Emily Witcraft
LAB TIME/DATE: 1/22/2013

Positive and Negative Controls
1. Why are there a number of washing steps in serological tests?
To make sure that all of the excessive antibodies off, and to prevent the nonspecific bindings of the antibodies and the antigens. 2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results?

When there is a positive result that has a negative control, and it can invalidate all of the results. 3. Describe how you would know that you had a “false negative” result. What does this mean for the rest of your results?

When there is a negative result that has a positive control, and it can invalidate all of the results. Direct Fluorescent Antibody Technique
4. Why is this technique a direct method?
The antibodies have a fluorescent dye.
5. What is an elementary body?
An infectious cell type or a particle of several microorganisms. 6. How do elementary bodies look under the fluorescent microscope?
It has some darker spots, some green patches, a red color, and various in the shape. 7. What do you think would happen if you did not fix the sample to the slide with 95% ethyl alcohol?
It fixes the sample, and it prevents the samples from being washed off. If it wasn’t used the same would just wash off. 8. Which patient(s) tested positive for Chlamydia?
Patients two and four.
9. Was there any nonspecific binding for any of the samples? Explain.

Yes, patient three had one elementary body that had green color out of the 26 bodies. Ouchterlony Technique
10. What is a precipitin line?
When the antigen and the antibody are in the optimal proportions, crosslinking the antigen and the antibody then occurs, which forms the insoluble precipitation. 11. What is the unknown antigen in the simulation?

12. Considering your results, do you think that human serum albumin and bovine serum...
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